南方医科大学学报2011，Vol.31Issue(3)：499-503,5.
Gluc-Fluc双荧光素酶质粒转染MB49细胞后荧光表达特性
Expression of Gluc-Fluc dual luciferase plasmid after transfection into MB49 bladder cells
摘要
Abstract
To investigate the expression characteristics of Gluc-Fluc dual luciferase plasmid after its transfection into MB49 bladder cells. Methods pAAV2neoCAG-Gluc-2A-Fluc and pAAV2neo-Gluc plasmids were separately transfected into MB49 cells via LipofectamineTM2000. The Glue activity in the cell culture supernatant and the Flue activity in the cells were detected by luminometer and Lumina Imaging system. Results The luminometer result showed that the activity of Glue in the supernatant increased gradually in a cell number- and time-dependent manner, while Flue activity in the cells increased with the cell number but not with time. The Lumina Imaging system showed that Gluc-Fluc was successfully expressed in MB49 bladder cells and cell lines with stable Gluc-Fluc expression were obtained after G418 selection. Conclusion Glue in the dual luciferase plasmid retains its expression characteristics. Due to the advantages of Flue in localization in living imaging and the easy quantitative detection of Glue, the dual luciferase plasmid, after transfection in MB49 bladder cells, allows reliable and dynamic detection of tumor growth in animal models.关键词
双荧光索酶/转染/活体成像/膀胱癌Key words
dual luciferase plasmid/ transfection/ living imaging/ bladder cancer分类
生物科学引用本文复制引用
张信基,石小军,孙鹏宇,张哲欢,付昕阳,谭万龙..Gluc-Fluc双荧光素酶质粒转染MB49细胞后荧光表达特性[J].南方医科大学学报,2011,31(3):499-503,5.基金项目
广东省自然科学基金(9151051501000030) （9151051501000030）
