南方医科大学学报2011，Vol.31Issue(3)：512-517,6.
脂肪组织源性干细胞分步诱导分化为神经元样细胞
A two-step protocol for induction of rat adipose tissue-derived stem cells into neuron- like cells
摘要
Abstract
To investigate the differentiation potential of rat adipose tissue-derived cells (ADSCs) into neuron-like cells in vitro using a two-step induction protocol. Methods ADSCs isolated from the epididymal fat pads in male SD rats by means of differential attachment were cultured in vitro and subjected to adipogenic induction. After flow cytometric identification of the cell surface antigens CD106, CDllb, CD45, CD49d, CD90 and CD29, the third-passage ADSCs were induced to transdifferentiate into neural stem cell (NSC)-like cells in DMEM/F12 medium containing 10 ng/ml basic fibroblast growth factor (bFGF), 20 ng/ml epidermal growth factor (EGF) and 2% B27. The resultant NSC-like cells were then induced to differentiate into neuron-like cells in the neurobasal medium containing 10 ng/ml brain-derived neurotrophic factor (BDNF), 10 ng/ml glial cell line-derived neurotrophic factor (GDNF) and 1 (omol/L retinoic acid (RA). Imrnunocytochemistry was employed to identify the expression of the cell surface markers nestin, MAP2 and NeuN. Results The isolated ADSCs were positive for CD90 and CD29, and oil red O staining of the induced adipose-like cells yielded positive results. The third-passage ADSCs induced for 7 days aggregated as floating cell spheres positive for NSC surface antigen nestin. Further induction in neurobasal medium for 4 h resulted in adhesion of the cell spheres and the formation of cell processes extending from some peripheral cells, suggesting a morphological resemblance to neurons. Most of the cells showed positivity for MAP2 and NeuN. Conclusion ADSCs can be induced to differentiate into neuron-like cells in vitro under appropriate conditions.关键词
脂肪组织源性干细胞/分化/神经干细胞/神经元Key words
adipose tissue-derived stem cells/ differentiation/ neural stem cells/ neurons分类
生物科学引用本文复制引用
陈盼盼,张丽华,董为人,刘杰明,张易,乔伟光,陈英华,赵姝,郭家松..脂肪组织源性干细胞分步诱导分化为神经元样细胞[J].南方医科大学学报,2011,31(3):512-517,6.基金项目
广东省高校优秀中青年人才基金(C1010086) （C1010086）
佛山市科技攻关项目(201008063) （201008063）
佛山市卫生局医学科研立项(2010177) （2010177）
