南方医科大学学报2011，Vol.31Issue(3)：535-538,4.
体外循环与非体外循环冠状动脉旁路移植术循环内皮细胞损伤的研究
Circulating endothelial cell injury in on-pump and off-pump coronary-artery bypass grafting
宋铁牛 1赵启明 1高秉仁1
作者信息
- 1. 兰州大学第二医院心外科,甘肃,兰州,730030
- 折叠
摘要
Abstract
To investigate the difference in circulating endothelial cell (CECs) injuries following on-pump and off-pump coronary-artery bypass surgery. Methods We randomly assigned 48 patients scheduled for urgent or elective coronary artery bypass grafting into two groups to receive on-pump and off-pump procedures. Blood samples were obtained before anesthesia, at the end of operation, and on days 1 and 3 after the operation, and CECs were isolated and counted using dynabeads coated with the specific antibody of CD146. Single cell gel electrophoresis was used to observe the morphological changes of the CECs. Results In the cardiopulmonary bypass (CPB) group, the number of CECs was significantly greater than that in non-CPB group (P0.05) at the end of surgery and 1 day after the operation. On postoperative day 3, the number CECs was similar between the two groups (P>0.05). The length of the comet tail was longer in CPB group with stronger fluorescence intensity than in the non-CPB group. Conclusion Compared with of-pump coronary artery bypass grafting, on-pump coronary artery bypass grafting results in more serious CEC injury, which is closely related to the prognosis.关键词
血管内皮细胞/冠心病/体外循环Key words
circulating endothelial cells/ coronary heart disease/ cardiopulmonary bypass分类
医药卫生引用本文复制引用
宋铁牛,赵启明,高秉仁..体外循环与非体外循环冠状动脉旁路移植术循环内皮细胞损伤的研究[J].南方医科大学学报,2011,31(3):535-538,4.
