现代妇产科进展2011，Vol.20Issue(3)：225-228,4.
三氧化二砷对人卵巢癌SKOV3细胞侵袭转移能力的影响
The effect of aresenic trioxide on invasion, metastasis of human ovarian cancer cell line SKOV3
陈学娟 1张向宁 1王凤琴 1许昭君 1贾宗洋 1靳艳慧1
作者信息
- 1. 山东大学齐鲁医院妇产科,济南,250012
- 折叠
摘要
Abstract
Objective :To explore the effects of aresenic trioxide ( As2O3) on the invasion and metastasis to SKOV3 cells and the expression of urokinase type plasminogen activator ( uPA) and it's receptor ( uPAR). Methods: SKOV3 cells were treated with 0. 5μmol/L,1μmol/L and 2μmol/L As2O3 for 48 hours respectively. The suppressing effect on the invasion and metastasis to SKOV3 cells was detected by Transwell invasion chamber. The expression level of uPA and uPAR mRNA and protein were detected by real-time quantitative PCR and SABC immune-tissue-chemistry method. Results: The number of cells which through the simulative basement membrane is decreased dfter treated by As2O3. The invasion and metastasis ability of passing the transwell invasion chamber decreased in SKOV3 cells which treated by As2O3( P＜0. 05 ) , and the expression level of uPA , uPARmRNA and protion of SKOV3 cells were lower in companson with untreated cells in a dosage-dependent manner( P＜0. 05 ) . Conclusion: SKOV3 cells,invasion and metastasis ability can be inhibited by As2O3. The mechanism might be associated with the reduction of uPA and uPAR expression.关键词
卵巢肿瘤/三氧化二砷/侵袭/转移/尿激酶型纤溶酶原激活物/尿激酶型纤溶酶原激活物受体Key words
Ovarian neoplasms / Aresenic trioxide / Invasion / metastasis / Urokinase type plasminogen activator/ uPAR分类
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陈学娟,张向宁,王凤琴,许昭君,贾宗洋,靳艳慧..三氧化二砷对人卵巢癌SKOV3细胞侵袭转移能力的影响[J].现代妇产科进展,2011,20(3):225-228,4.
