现代科学仪器Issue(1)：73-77,5.
农产品质量安全快速检测技术——微波辅助水解比色法快速测定动物水解蛋白中羟脯氨酸的研究
Rapid Detection Technology of Agricultural Products for Quality and Safety --Rapid Determination 4-Hydroxyproline of Hydrolyzed Collagen by Microwave-assisted Hydrolysis and Colorimetric Method
摘要
Abstract
A rapid determination method for 4-Hydroxyproline of hydrolyzed collagen was developed, based on microwave-assisted hydrolysis and colorimetric method.In this paper the influence effects were investigated, such as microwave-assisted hydrolysis conditions (the concentration of H2SO4, volume of H2SO4, hydrolysis time and microwave power) and color development conditions (oxidation time, oxidation temperature, color developing time).The results showed that microwave-assisted hydrolysis had the same precision and accuracy compared to traditional hydrolysis method.However, the hydrolysis time by microwave-assisted hydrolysis was reduced from 16 h to 4～8 min.The novel rapid analyzer and reagent kit for 4-Hydroxyproline designed by Jilin University - Little Swan Instruments Co., Ltd.was used to detect 4-Hydroxyproline.It was found that at the concentration range of 0.10×10-3 g/L～2.50×10-3 g/L, the absorbance and concentration of 4-Hydroxyproline has a good linear relationship, and the recovery rate between 90％ and 105％.关键词
动物水解蛋白/微波辅助水解/快速检测仪Key words
Hydrolyzed collagen/Microwave-assisted hydrolysis/Rapid detection analyzer分类
生物科学引用本文复制引用
丁毅,王兴华,郭蔷微,宋丹,高德江,于永..农产品质量安全快速检测技术——微波辅助水解比色法快速测定动物水解蛋白中羟脯氨酸的研究[J].现代科学仪器,2011,(1):73-77,5.基金项目
质检公益性行业科研专项(200810474) （200810474）
国家科技支撑计划项目(2006BAJ02A07-3) （2006BAJ02A07-3）
