现代科学仪器Issue(1)：90-95,6.
肉制品中非法添加色素的高效液相色谱快速检测方法
Fast Determination of Banned Dyes in Meat Products by High Performance Liquid Chromatography
摘要
Abstract
An accurate and sensitive method based on the use of GPC-HPLC-DAD was devised for the simultaneous determination of Sudan ( Ⅰ ～ Ⅳ ), Sudan red B, Sudan red 7B, Sudan orange G and Para red in meat products.The GPC clean-up procedure was adopted to avoide some interference and damage of the column for quantification of 8 dyes in ham and sausage samples.For the separation, Kinetex C18 column was used and the mobile phase contained acetonitrile and 20mmol/L ammonium acetate solution at pH 4.5.The optimized subsection wavelength condition reduced the change of methods for determination of different dyes, these dyes were analyzed within 10 min.The 8 compounds behave linearly in the 0.05-50 mg/L concentration range, with correlation coefficient＞0.999.LODs and LOQs for the investigated dyes were in the ranges of 0.014-0.075 mg/kg and 0.045-0.25 mg/kg depending on matrices, respectively.The recoveries of the 8 dyes in two matrices ranged from 87.8-111 .9％.The illustrated method is suitable for routine qualitative and quantitative analyses of synthetic colorants in meat products.关键词
非法添加色素/凝胶渗透色谱/高效液相色谱/肉制品Key words
Banned dyes/ GPC/ HPLC-DAD/ Meat products分类
化学化工引用本文复制引用
王菡,储晓刚,曹远银..肉制品中非法添加色素的高效液相色谱快速检测方法[J].现代科学仪器,2011,(1):90-95,6.基金项目
北京市科委项目(食品中未知添加物质筛查确证技术研究),D08050200310803 （食品中未知添加物质筛查确证技术研究）
