动物学研究2011，Vol.32Issue(2)：179-187,9.DOI:10.3724/SP.J.1141.2011.02179
新疆草兔的种群遗传结构和亚种分化
Genetic structure and subspecies divergence of Lepus capensis in Xinjiang
摘要
Abstract
To date, the genetic structure and genetic diversity of Lepus capensis in Xinjiang has not been systematically studied at the molecular level, and its subspecies taxonomic status has been under debate for years.According to traditional morphology, there are three subspecies of L. capensis distributed in Xinjiang: L.c. centrasiaticus,L.c. lehmanni and L.c. pamirensis. In this study, we determined 592 bp D-loop sequences of 87 cape hares from Xinjiang Province. Forty-four haplotypes were defined based on 148 polymorphic sites. Both the haplotype diversity (0.977 ±0.005) and nucleotide diversity (0.064 ± 0.031) are high. Fs r P values are significantly high and no haplotype was shared among the four geographic populations, indicating that genetic differentiation among populations is significant. AMOVA shows that most of the genetic differentiation occurred among geographic groups, indicating that geographic isolation such as mountains and deserts might make an effective barrier against gene flow. Both the phylogenetic tree and median-joining network grouped 44 haplotypes into four distinct clades corresponding to four geographic areas,indicating an obvious phylogeographic pattern. Our data supported the subspecies status of L. c. lehmanni. The fact that haplotypes of L. c. centrasiaticus were grouped into two distinct clades suggests that this traditional subspecies should be considered as two subspecies. In addition, L. c. pamirensis shows a significantly higher sequence divergence compared to other subspecies, and the difference even reached the level of species.关键词
草兔/线粒体DNA/控制区/群体遗传多样性/亚种分化分类
生物科学引用本文复制引用
单文娟,刘江,马合木提·哈力克..新疆草兔的种群遗传结构和亚种分化[J].动物学研究,2011,32(2):179-187,9.基金项目
国家自然科学基金项目(31060152) （31060152）
新疆维吾尔自治区高校科研计划青年教师科研启动基金项目(XJEDU2008S03) （XJEDU2008S03）
新疆大学校院联合资助项目(XY080117) （XY080117）
新疆大学科研实训项目(XJU-SRT-10012) （XJU-SRT-10012）
