国际妇产科学杂志2011，Vol.38Issue(2)：148-154,后插2,封3,9.
莉芙敏对去卵巢大鼠下丘脑视前区5-HT1AR和5-HT2AR表达的影响
Effect of Remifemin on Expression of 5-HT1AR and 5-HT2AR in Preoptic Area of Hypothalamus of Ovariectomized Rats
摘要
Abstract
Objective : To investigate regional changes in 5-hydroxytryptamine ( 5-HT) 1A receptors (5 -HT1AR) and 5-hydroxytryptamine (5-HT) 2A receptors (5-HT2AR) in the OVX rat preoptic area of hypothalamus after 1-4 weeks treatment with isopropanolic black cohosh extract-remifemin (ICR). and explore its potential mechanism of alleviating hot flushes during the peri -menopausal period. Methods:The rats were divided into f'our groups, ovariectomy (OVX) group,sham group,ovariectomy with estradiol valerate (E2) treatment (OVX+E2)group, and ovariectomy with ICR treatment (OVX +ICR) group. The sham group underwent a sham operation without ovariectomies.while the other three groups underwent bilateral ovariectomies under sterile conditions. All rats were given 2 weeks to recover after the operation, then each group was given a different treatment lasting 1 week , 2 weeks , 3 weeks and 4 weeks. The expression of 5-HTIAR and 5-HT2AR in the rat preoptic area of hypothalamus(POAH)were detected by immunohistochemistry. Results : ①After 1-4 weeks' treatment . the number of 5-HT1AR positive cells and the optical density ( OD) in POAH of OVX group significantly increased compared with that in the same period of sham group ; while the number of 5-HTIAR positive cells and the optical density in periventricular zone of OVX+ICR group and OVX+E2 group significantly decreased compared with that in the same period of OVX group.②After 1-4 weeks'treatment,the number of 5-HT2AR positive cells and the optical density (OD) in intermediate zone and lateral zone of OVX group significantly increased compared with that in the same period of sham group. The number of 5-HT2AR positive cells and the optical density (OD) in intermediate zone and lateral zone of OVX+ICR group and OVX+E2 group significantly increased compared with that in the same period of OVX group after 1 -2 weeks' treatment. While the result is reverse after 3 -4 weeks' treatment. Conclusions:Ovariectomized rats had increased expression of 5 -HT1AR and 5 -HT2AR.After treatment with remifemin or estradiol valerate.the expression of 5-HT1AR in POAH significantly decreased. While the expression of 5 -HT2AR in POAH decreased following an increased period.Regulating the expression of 5 -HT1AR and 5 -HT2AR in POAH may be one of the mechanisms of remifemin alleviating menopausal hot flush .关键词
升麻属/雌激素类/受体,血清素,5-HT1A/受体,血清素,5-HT2A/免疫组织化学/大鼠,Sprague-DawleyKey words
Cimicifuga/ Estrogens / Receptor, serotonin , 5 -HT1A / Receptor, serotonin, 5 -HT2A / Immunohistochemistry / Rats , Sprague-Dawley引用本文复制引用
马晓艳,张慧,王珂,杨立元,朱赛楠,濮鸣亮,秦丽华,白文佩..莉芙敏对去卵巢大鼠下丘脑视前区5-HT1AR和5-HT2AR表达的影响[J].国际妇产科学杂志,2011,38(2):148-154,后插2,封3,9.基金项目
国家自然科学基金(81070462). （81070462）
