江西农业大学学报2011,Vol.33Issue(2):350-354,5.
黑龙江茴鱼(Thymallus arcticus grubei Dybowski)的细胞遗传学分析
Cytogenetic Analysis of Thymallus arcticus grubei Dybowski
摘要
Abstract
Metaphase chromosome specimens of Thymallus arcticus grubei Dybowsk injected with PHA and colchicin were prepared from short - term culture of kidney cells with air - drying technique. It' s diploid chromosome number was 2n = 92. The karyotype was composed of 36m, lOsm, 8st and 38t chromosomes, NF = 138. It's diploid nucleus DNA content was measured from the somatic cell of T. a. grubei using flow cytometer( Beckman cellLab Quant, made in USA) and the DNA content (2.3 pg/N) of erythrocytes of chick ( Callus sp) as standard. The T. a. grubei DNA content was 5.03 pg/N. According to the present results and reported date, T. a. grubei is possibly an tetraploid(4n).关键词
黑龙江茴鱼/染色体/核型/DNA含量分类
农业科技引用本文复制引用
薛淑群,尹洪滨,赵吉伟..黑龙江茴鱼(Thymallus arcticus grubei Dybowski)的细胞遗传学分析[J].江西农业大学学报,2011,33(2):350-354,5.基金项目
科技部科技基础专项基金项目(2006DKA30470-005)、黑龙江水产研究所基本科研业务费专项资金(2007HSYZX-YZ-01)和黑龙江水产研究所科研专项基金(2009HSYZX-SJ-06) (2006DKA30470-005)