西北林学院学报2011，Vol.26Issue(1)：128-130,3.
刺槐根系可溶性蛋白SDS-PAGE分离方法的改进
Methodological Improvement of Seperating Soluble Protein by SDS-PAGE from Robinia pseudoacacia Root
摘要
Abstract
An improvement was made in the conventional method SDS-PAGE for separating soluble protein from the roots of Robinia pseudoacacia by increasing the content of SDS, β-mercaptoethanol and glycerine to 50 ％.The separating effect of improved method was much better than the conventional one.Causes of the improvement were discussed: in the conventional method, the concentrations of SDS and β-mercaptoethanol were not high enough to completely dissolve the soluble protein, and the concentration of glycerine was also not high enough to precipitate the sample.关键词
刺槐根系/可溶性蛋白/SDS-PAGE/改进分类
农业科技引用本文复制引用
陈明涛,赵忠,权金娥..刺槐根系可溶性蛋白SDS-PAGE分离方法的改进[J].西北林学院学报,2011,26(1):128-130,3.基金项目
国家自然科学基金项目(30671673) （30671673）
