激光生物学报2011，Vol.20Issue(2)：261-268,8.DOI:10.3969/j.issn.1007-7146.2011.02.022
一株毒死蜱降解新菌株Sphingopyxis terrae R17的分离鉴定及降解特性
Isolation, Identification and Chlorpyrifos Degradation Characteristic of New Chlorpyrifos Degrading Strain Sphingopyxis terrae R17
摘要
Abstract
One bacterial strain, named R17, was isolated from the sludge polluted with chlorpyrifos which had the capability to utilize chlorpyrifos as the sole carbon and energy sources. Strain R17 was identified preliminarily as Sphingopyxis terrae based on its physiological and biochemical characters and its 16S rDNA homologue sequence analysis. Under the optimal growth condition, 35 ℃ and pH 7 ～8. Strain R17 could reach 9.18 × 108 cfu/mL after 28 h. The chlorpyrifos degradation characteristics of strain RI7 were studied with different times and different initial chlorpyrifos concentrations. The results showed that with the inoculation of 9.18 × 107 cfu/mL, the degradation rate of chlorpyrifos by strain RI7 were 18.59 ％, 31.23 ％, 36.55 ％ and 47.69 ％ in 1 d, 2 d, 3 d and 4 d respectively. The degradation rate of R17 with initial concentration of 1 mg/L, 5 mg/L, 10 mg/L chlorpyfifos reached 50.21％, 43.46 ％ and 31.23 ％ after 2 d, respectively.关键词
毒死蜱/Sphingopyxis terrae/分离鉴定/降解特性Key words
chlorpyrifos/ Sphingopyxis terrae/ isolation and identification/ degradation characteristics分类
资源环境引用本文复制引用
李瑞雪,花日茂,唐欣昀,王昀璐,张君,王道胜..一株毒死蜱降解新菌株Sphingopyxis terrae R17的分离鉴定及降解特性[J].激光生物学报,2011,20(2):261-268,8.基金项目
国家"863"高技术研究发展计划项目(2007AA102403) （2007AA102403）
安徽省科技攻关项目(08010302166) （08010302166）
国家科技支撑计划项目(2007BAD87B06) （2007BAD87B06）
