基础医学与临床2011，Vol.31Issue(4)：426-429,4.
尿酸抑制人脐静脉内皮细胞合成一氧化氮
Uric acid inhibits the synthesis of nitric oxide in human umbilical vein endothelial cells
王琦 1曾学军 1方卫纲 1陈连凤 2何敛3
作者信息
- 1. 中国医学科学院北京协和医学院北京协和医院普通内科,北京,100032
- 2. 中国医学科学院北京协和医学院北京协和医院心内科,北京,100032
- 3. 拜耳医药保健有限公司全球药政事务部,美国,新泽西州,NJ,07045
- 折叠
摘要
Abstract
Objective To investigate the effect of uric acid (UA) on the expression of endothelial nitric oxide synthase (eNOS) and the production of nitric oxide in cultured human umbilical vein endothelial cells (HUVECs).Methods HUVECs were incubated with different concentrations of UA (0,0.5,1,1.5,2 mg/L) and 50 mg/L oxLDL( as positive control) for 24, 48 and 72 h.Then, eNOS mRNA was detected by RT-PCR.The expression of eNOS protein was observed by Western blot.NO in supernatant medium was analyzed by enzymic method.Results UA 5 mg/dl group increased the expression of eNOS mRNA of HUVECs as compared with that from placebo control group( P ＜0.05 ).While the concentration of UA increasing and time runing, the expression of eNOS and the production of NO and ox-LDL decreased significantly(72 h NO2 -/NO3 - is 0.52 ±0.18 versus 1.00 ±0.1 for 2 mg/L versus control, P ＜ 0.05 ).Conclusion UA inhibits the expression of eNOS and the production of NO of HUVECs in a dose-dependent and time-dependent manner.It suggests that UA may damage the function of endothelial cells and may influence the genesis and development of cardiovascular diseases.关键词
尿酸/内皮功能/一氧化氮合成/一氧化氮Key words
uric acid/ endothelial disfunction/ edothelial nitric oxide synthase/ nitric oxide分类
医药卫生引用本文复制引用
王琦,曾学军,方卫纲,陈连凤,何敛..尿酸抑制人脐静脉内皮细胞合成一氧化氮[J].基础医学与临床,2011,31(4):426-429,4.
