应用生态学报2011，Vol.22Issue(3)：727-733,7.
转Bt基因水稻根际土壤微生物多样性的磷脂脂肪酸(PLFAs)表征
Microbial diversity in rhizosphere soil of transgenic Bt rice based on the characterization of phospholipids fatty acids
摘要
Abstract
Taking non-transgenic parental rice as the control, and by using 13C pulse-chase labeling method coupled with phospholipid fatty acid ( PLFA) analysis, this paper studied the effects of transgenic Bt rice on the microbial diversity in thizosphere soil. The results showed that in the rhizosphere soils of transgenic Bt rice and its non-transgenic parent, saturated PLFAs and branched PLFAs were the main. followed by monounsaturated PLFAs. and polyunsaturated PLFAs. A significantly lower amount of Gram-positive bacterial PLFAs and a higher amount of Gram-negative bacterial PLFAs were observed in the thizosphere soil of transgenic Bt rice at its seedling, booting, and heading stages, as compared with the control. In the whole growth period of rice. transgenic Bt gene had no significant effects on the fungal and actinomycete PLFAs in thizosphere soil. and no significant difference was observed in the thizosphere soil l3C-PLFA content between transgenic Bt rice and its non-transgenic parent. These findings indicated that the insertion of exogenous cryIAb gene into rice only had temporary effects on the microbial community composition in the thizosphere soil of rice.关键词
转Bt基因水稻/13C标记/磷脂脂肪酸/根际土壤微生物分类
生物科学引用本文复制引用
刘微,王树涛,陈英旭,吴伟祥,王璟..转Bt基因水稻根际土壤微生物多样性的磷脂脂肪酸(PLFAs)表征[J].应用生态学报,2011,22(3):727-733,7.基金项目
河北省教育厅科学研究计划项目(z2010228)资助. （z2010228）
