中国组织工程研究与临床康复2011，Vol.15Issue(15)：2769-2772,4.DOI:10.3969/j.issn.1673-8225.2011.15.026
脑源性神经营养因子与心脏微血管内皮细胞的管状形成
Brain derived neurotrophic factor promotes tube formation of cardiac microvascular endothelial cells
摘要
Abstract
BACKGROUND:It has been discovered that brain derived neurotrophic factor (BDNF) can promote endothelial cell survival and induce angiogenesis. However, the cellular and molecular mechanism regarding to BDNF-induced angiogenesis is still unknown. OBJECTIVE:To observe the effect of BDNF on tube formation of cardiac microvascular endothelial cells (CMECs) in three-dimensional (3D) in vitro tube formation assay.METHODS:Rat CMECs were isolated and cultured until they formed a single spheroid. These spheroids were embedded in the mixture of collagen type Ⅰ -carboxymethylcellulose (CMC). Then, 50 μg/L, 70 μg/L, 100 μg/L BDNF was added into the wells respectively. The number of branch and the cumulative length of all capillary-like structure were analyzed in 24 and 48 hours after BDNF treated respectively.RESULTS AND CONCLUSION:The capillary-like structure for each CMECs spheroid was visible in 3D collagen type Ⅰ -CMC containing after 24 hours culture. It was found that BDNF could promote tube formation of CMECs. The cumulative length and the branch of capillary-like structure of 100 μg/L BDNF group was significantly longer than that other groups respectively. The cumulative length of CMECs spheroids was significantly longer after 48 hours compared with at 24 hours. The results of this study suggested that BDNF could promote tube formation of CMECs in 3D in vitro tube formation assay. The promoting effect of BDNF is dosage dependence.关键词
心脏微血管内皮细胞/脑源性神经营养因子/血管新生/管状结构形成分类
医药卫生引用本文复制引用
刘少坤,曹亮,张靓,沈晓涛,赵宝寅,齐绪峰,秦俊文,蔡冬青..脑源性神经营养因子与心脏微血管内皮细胞的管状形成[J].中国组织工程研究与临床康复,2011,15(15):2769-2772,4.基金项目
863项目(2007AA02Z105) （2007AA02Z105）
国家自然科学基金(30770886,30570369,30340038,30973158) （30770886,30570369,30340038,30973158）
广东省自然科学基金重点项目(04105826) （04105826）
广东省科技攻关项目(2004B30601007) （2004B30601007）
广东省国际合作项目(2009B050900007) （2009B050900007）
中央高校基本科研业务费专项资金(21609408). （21609408）
