中国妇幼健康研究2011，Vol.22Issue(3)：293-295,3.DOI:10.3969/j.issn.1673-5293.2011.03.014
重复应用TGF-β1对缺氧缺血性新生大鼠的影响
Effects of repeated TGF-β1 on neonatal rats with hypoxic-ischemic brain damage
摘要
Abstract
To investigate the effects of TGF-β1 on neonatal rats with hypoxic-ischemic brain damage ( HIBD ). Methods Ninety-five healthy unsexed 7-day-age SD neonatal rats were randomly divided into four groups, normal control ( NC ) group, sham operation group, hypoxic-ischemic ( model ) group, treated group ( including saline-treated group and TGF-β1-treated group ). After the HIBD model was performed, treated group was injected with 10ng saline or TGF-β1 by abdominal cavity in 2 hours and 10 days. The pyramidal cell apoptosis and dynamic expression of Caspase-3 in brain tissue CA1 were examined in 10 days and 40 days with the methods of TUNEL and ElivisionTM plus immunohistochemistry. Results Ten days after HIBD, apoptosis of pyramidal cell( t＝ 9.35, P ＜ 0.05 ) and expression of Caspase-3( t＝ 7.43,P ＜ 0.05 ) existed in TGF-β1 treated group compared with NC group and sham operation group, while the apoptosis decreased( t＝ 5.63,P ＜ 0.05 ) and the expression of Caspase-3 reduced( t＝ 4.55, P ＜ 0.05 ) compared with model group and saline-treated group. Forty days after HIBD, apoptosis of pyramidal cell existed( t＝ 7.25, P ＜ 0.05 ) and expression of Caspase-3 ( t＝8.17,P ＜ 0.05 )could be detected in model group, and the apoptosis still existed ( t＝ 6.07, P ＜ 0.05 ) and the expresstion of Caspase-3 could be found ( t＝ 3.98 ,P＜0.05 ) in TGF-β1 treated group compased with NC group and sham operation group. Conclusion There are significant protective effects on neonatal rats with HIBD after TGF-β1 treatment. Single dosage of TGF-β1 can protect neuron, but repeated use of TGF-β1 doesn't prolong the protective effects. TGF-β1 might act by depressing the expression of Caspase-3 which is related with apotosis. The experiment provides some theoretic basis for clinical use of TGF-β1 in treating HIBD, and offers a clue for future research on mechanism of TGF-β1 in treating brain damage.关键词
转化生长因子-β1/缺氧缺血/脑/新生SD大鼠Key words
TGF-β1 /hypoxia-ischemia/ brain/ neonatal SD rats分类
医药卫生引用本文复制引用
李小权,张菊平,刘俐,刘黎明..重复应用TGF-β1对缺氧缺血性新生大鼠的影响[J].中国妇幼健康研究,2011,22(3):293-295,3.基金项目
陕西省科技攻关资助项目(NO.20006K14-G12) （NO.20006K14-G12）
