中国组织工程研究与临床康复2011，Vol.15Issue(14)：2495-2499,5.DOI:10.3969/j.issn.1673-8225.2011.14.006
白细胞介素6体外诱导骨髓间充质干细胞向肝细胞的分化
Interleukin 6 induces the differentiation of bone marrow mesenchymal stem cells into hepatocytes in vitro
摘要
Abstract
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are an important source of hepatocytes. BMSCs can differentiate into hepatocytes under the induction of multiple cytokines and growth factors, and participate in repair and reconstruction of liver function.OBJECTIVE: To explore the effect of interleukin 6 on the differentiation of BMSCs into hepatocytes.METHODS: BMSCs were isolated from Kunming male mice and cultured by attachment method and treated with interleukin 6(2.5, 5, 10, 20 μg/L) in HEPATOZYME-SFM nutrient solution to induce the BMSCs differentiation into hepatocytes. On days 0, 7,14, 21 , 28 after induction, the expressions of cytokeratin 18, alpha-fetoprotein and glycogen were identified by immunohistochemical method and periodic acid-schiff. The contents of albumin and nitric oxide in the liquid supernatant were identified by ELISA and nitrate reductase method, respectively.RESULTS AND CONCLUSION: Interleukin 6 resulted in dose-dependent (2.5, 5, 10 μg/L) and time-dependent increase in the expressions of cytokeratin 18 and glycogen and the content of albumin. The expression of alpha-fetoprotein increased then decreased to disappearance on day 21 . The above effects of interleukin 6 (20 μg/L) was weaker than that of interleukin 6(10 μg/L). The content of small amount nitric oxide was detected when interleukin 6 was 10 μg/L on day 28. These demonstrated that interleukin 6 is able to induce the differentiation of BMSCs into hepatocyte-like cells, which shows strongest effects at 10 μg/L.Increased mass concentration showed decreased induction effects.关键词
骨髓间充质干细胞/白细胞介素6/分化/肝细胞/干细胞培养与分化分类
基础医学引用本文复制引用
宋丽华,王庸晋,王金胜,黄燕,武翠玲,石变华..白细胞介素6体外诱导骨髓间充质干细胞向肝细胞的分化[J].中国组织工程研究与临床康复,2011,15(14):2495-2499,5.基金项目
山西省高校科技研究开发项目(200611034),长治医学院科研发展基金项目(200715). （200611034）
