中国神经再生研究（英文版）2011，Vol.6Issue(12)：885-891,7.DOI:10.3969/j.issn.1673-5374.2011.12.001
The Chinese herbal formula Tongluo Jiunao promotes expression of brain-derived neurotrophic factor/tropomyosin-related kinase B pathways in a rat model of ischemic brain injury
The Chinese herbal formula Tongluo Jiunao promotes expression of brain-derived neurotrophic factor/tropomyosin-related kinase B pathways in a rat model of ischemic brain injury
Peiman Alesheikh 1Yangyang Yan 1Huiling Tang 1Pengtao Li 1Wei Zhang 1Yanshu Pan 1Arezou Mashoufi 1Liyun Zhao 1Runjun Wang 1Bo Di1
作者信息
摘要
Abstract
The neurotrophin-Trk receptor pathway is an intrinsic pathway to relieve damage to the central nervous system. The present study observed the effects of Tongluo Jiunao (TLJN), which comprises Panax Notoginseng and Gardenia Jasminoides, on expression of brain-derived neurotrophic factor (BDNF) and tropomyosin-related kinase B (TrkB) in a rat model of focal cerebral ischemic injury. Xue Sai Tong (XST), comprising Panax Notoginseng, served as the positive control. Mechanisms of neuroprotection were analyzed following TLJN injection. Following establishment of the middle cerebral artery occlusion models, TLJN and XST were intraperitoneally injected, and 2, 3, 5-triphenyltetrazolium chloride staining results revealed that TLJN injection reduced infarct volume, suggesting that TLJN exerted a neuroprotective effect. Enzyme-linked immunosorbent assay results showed that TLJN elevated BDNF and growth associated protein-43 expression in ischemic brain tissues, as well as serum BDNF levels. Reverse-transcription polymerase chain reaction and western blot results showed that TLJN injection did not affect TrkB expression in the ischemic brain tissues of rats. These results suggested that TLJN injection reduced damage to ischemic brain tissues and increased BDNF expression. In addition, TLJN injection resulted in better promoting effects on neurotrophic factor expression compared with XST.关键词
brain-derived neurotrophic factor/tropomyosin-related kinase B/Tongluo Jiunao injection/ischemic strokeKey words
brain-derived neurotrophic factor/tropomyosin-related kinase B/Tongluo Jiunao injection/ischemic stroke引用本文复制引用
Peiman Alesheikh,Yangyang Yan,Huiling Tang,Pengtao Li,Wei Zhang,Yanshu Pan,Arezou Mashoufi,Liyun Zhao,Runjun Wang,Bo Di..The Chinese herbal formula Tongluo Jiunao promotes expression of brain-derived neurotrophic factor/tropomyosin-related kinase B pathways in a rat model of ischemic brain injury[J].中国神经再生研究（英文版）,2011,6(12):885-891,7.
