中国组织工程研究与临床康复2011，Vol.15Issue(16)：2857-2861,5.DOI:10.3969/j.issn.1673-8225.2011.16.003
自制羊膜载体复合物植入表皮细胞修复全层皮肤缺损
Self-made amniotic carrier complex implanted with epidermal cells for full-thickness skin defects
富玲 1宋宁 1苏学忠 2于宁 1孙佩杰 3李娜然 3杨晓霞4
作者信息
- 1. 沈阳医学院附属奉天医院手外科,辽宁省沈阳市,110024
- 2. 沈阳医学院,辽宁省沈阳市110034
- 3. 沈阳医学院形态中心,辽宁省沈阳市,1100344
- 4. 沈阳医学院病理教研室,辽宁省沈阳市,110034
- 折叠
摘要
Abstract
BACKGROUND:The composite skin developed by using tissue engineering and cell culture technology is still not an ideal artificial skin, which is limited by weak local skin, poor tensile strength and resistance to friction and no skin appendages.OBJECTIVE:To observe the effect of basic fibroblast growth factor and vitamin C epidermal cell composite transplantation on full-thickness skin defects and to investigate the optimal time promoting the epidermal growth.METHODS:A total of 36 New Zealand white rabbits were prepared for three full-thickness skin defect models at the back. The self-made amnion carrying composite with basic fibroblast growth factor and vitamin C, pure amnion carrying composite, pure capping were implanted into the three defects, and were randomly divided into three groups. Epidermal cells were implanted into the site in related groups after 7, 14, and 21 days. After 7 days, general observation, histological observation,immunohistochemical observation were carried out.RESULTS AND CONCLUSION:The groups with autologuos epidermal cells implantation at 21 days had a better effect that those with autologous epidermal cells implantation at 14 and 7 days. Self-made amniotic carrier complex with basic fibroblast growth factor and vitamin C was superior to pure amnion carrying composite and pure capping. Basic fibroblast growth factor and vitamin C promoted the regeneration and renascence of the dermis. The latter of the dermis repairing and the former of the dermis restoring were the best stage which accelerates the renascence and regeneration of the epidermis.关键词
羊膜载体复合物/表皮细胞/碱性成纤维细胞生长因子/皮肤创伤愈合/真皮分类
医药卫生引用本文复制引用
富玲,宋宁,苏学忠,于宁,孙佩杰,李娜然,杨晓霞..自制羊膜载体复合物植入表皮细胞修复全层皮肤缺损[J].中国组织工程研究与临床康复,2011,15(16):2857-2861,5.
