中国组织工程研究与临床康复2011，Vol.15Issue(16)：2862-2866,5.DOI:10.3969/j.issn.1673-8225.2011.16.004
透明质酸钠溶液复合可注射型骨髓间充质干细胞修复退变椎间盘
Effect of injectable bone mesenchymal stem cells compounded with sodium hyaluronate in repairing disc degeneration
陈国仙 1王万明 2王国荣 1林宗锦 1李国山 1林智军 3刘航涛3
作者信息
- 1. 莆田市第一医院骨科,福建省莆田市,351100
- 2. 解放军南京军区福州总医院骨科,福建省福州市,350025
- 3. 解放军南京军区福州总医院附属第一医院,福建省莆田市,351100
- 折叠
摘要
Abstract
BACKGROUND:Sodium hyaluronate, as the matrix material of intervertebral disc tissue engineering scaffolds, can provide proteoglycan attachment points and increase deposition of proteoglycan, to make enough large porosity allowing the seed cell ingrowth.OBJECTIVE:To observe the effect of bone mesenchymal stem cells (BMSCs) mixed with sodium hyaluronate solution in repair of disc degeneration in rabbits.METHODS:The animal mode of degenerative intervertebral disc was built in rabbits by aspirating the nucleus pulposus (L1-2 and L3-4) through an posterolateral approach. Two weeks later, the mixtu re of BMSCs and sodium hyaluronate were injected into L3-4discs using micro-injection syringe as study group, while sodium hyaluronate alone was injected into L1-2 as control group. At 2, 4,8, 12 weeks after injection, the rabbit intervertebral disc was determined by plain radiography and histology.RESULTS AND CONCLUSION:The intervertebral disc height was reduced in control group, and gradually reduced in experiment group at 2 weeks after injection and then raised. There were significant differences in disc height index between two groups (P ＜0.05). Pathological examination showed the structure of the inner annulus fibrosus was significantly preserved in the experiment group, and the boundary between the nucleus and annulus could be clearly visualized. The implanted BMSCs proliferated and the pathological grading scores were significantly higher than control group. Expression of type Ⅱ collagen was significantly greater in the experiment group than in control group (P ＜ 0.05). BMSCs transplanted into intervertebral discs are shown to well survive and proliferate. The mixture of BMSCs and sodium hyaluronate could decelerate disc degeneration and repair the degenerated intervertebral discs.关键词
透明质酸钠/骨髓间充质干细胞/椎间盘退变/细胞移植/髓核抽吸分类
医药卫生引用本文复制引用
陈国仙,王万明,王国荣,林宗锦,李国山,林智军,刘航涛..透明质酸钠溶液复合可注射型骨髓间充质干细胞修复退变椎间盘[J].中国组织工程研究与临床康复,2011,15(16):2862-2866,5.
