中国组织工程研究与临床康复2011，Vol.15Issue(16)：2867-2870,4.DOI:10.3969/j.issn.1673-8225.2011.16.005
海藻酸钠复合载体长期培养软骨细胞的生物学稳定性
Biological stability of long-term culture chondrocytes on three-dimensional culture system of multifactorial sodium alginate
摘要
Abstract
BACKGROUND:Previous studies demonstrated that the survival time of chond rocytes is prolonged in three-dimensional culture system of multifactorial sodium alginate; extracellular matrix has a strong secretion.OBJECTIVE:To observe the effect of biological stability of long-term culture chondrocytes on three-dimensional culture system of multifactorial sodium alginate.METHODS:Chondrocytes were implanted on three-dimensional culture system which comprises sodium alginate, hyaluronic acid, chitosan, fibronectin, and basic fibroblast growth factor (bFGF), and the plane culture as controls. The morphological changes, differences of cell growth curves, and the contents of glycosaminoglycan (GAG) of cells supernatant fluid of chondrocytes were regularly observed in two different culture systems.RESULTS ANS CONCLUSION:Morphous of chondrocytes on three-dimensional culture system of multifactorial sodium alginate grew well and flourishingly, and formed the globular group of cells, cell proliferation was active. Proliferation of chondrocytes on the culture system of multifactorial sodium alginate was slightly higher than that implanted on plane cultured 2 days. Proliferation of chond rocytes was significantly higher cultured 4 days on the culture system of multifactorial sodium alginate. Chondrocytes were keep stable proliferation at 6-14 days culture; while the proliferation of chond rocytes cultu red on plane was gradually decrease. The contents of GAG of cells supernatant fluid of the three-dimensional culture system were significantly higher than monolayer culture system. Chond rocytes on three-dimensional culture system of multifactorial sodium alginate can long-term culture and keep their biological stability.关键词
海藻酸钠/软骨细胞/培养/载体/生物学稳定性分类
医药卫生引用本文复制引用
朱振宗,梁伟国,沈雁,杨小红,戴丽冰,谭见容,梁佩红,张金丽,康宁..海藻酸钠复合载体长期培养软骨细胞的生物学稳定性[J].中国组织工程研究与临床康复,2011,15(16):2867-2870,4.基金项目
广东省卫生厅课题项目(2004553) （2004553）
2004 年广州市卫生局重点课题项目(2004Z005). （2004Z005）
