中国组织工程研究与临床康复2011，Vol.15Issue(15)：2672-2676,5.DOI:10.3969/j.issn.1673-8225.2011.15.004
模拟失重状态下成骨细胞胰岛素样生长因子1基因表达与降钙素的干预
Interventional effect of calcitonin on insulin like growth factor 1 gene expression in osteoblasts under simulated microgravity
苏嘉霖 1张桦 1杨理婷 2杨锐 1王脉桃 1黄震3
作者信息
- 1. 南方医科大学珠江医院,内分泌科,广东省广州市510282
- 2. 南方医科大学珠江医院,血液科,广东省广州市510282
- 3. 南方医科大学珠江医院,外科学教研室,广东省广州市510282
- 折叠
摘要
Abstract
BACKGROUND: Space flight, bed-ridden state of low-gravity load will cause bone metabolic disorder which includes bone absorption and bone formation abnormal, leading to bone loss, decreased bone mineral density. Researches have showed that calcitonin can promote osteoblastic activity and mRNA expression of insulin like growth factor 1 (IGF-1).OBJECTIVE: To observe the effects of calcitonin on osteoblastic activity and mRNA expression of IGF-1 in vitro under simulated microgravity.METHODS: Osteoblasts were collected from newborn SD mouse by means of double enzyme digestion. The osteoblasts were polymerized and encapsulated the cells inside alginate carriers in Cacl2 solution. All cells were assigned into normal gravity,simulated microgravity and simulated microgravity + calcitonin (10, 40 and 80 IU/L) groups. Osteoblasts were cultured for 72 hours in simulated microgravity and normal gravity in the three-dimensional rotating vessel. The proliferation and apoptosis of the osteoblasts and alkaline phosphatase (ALP) activity were detected, in addition, the expression of IGF-1 , ALP and osteocalcin were measured by RT-PCR.RESULTS AND CONCLUSION: Compared with the normal gravity group, the osteoblasts proliferation rate and secretion of ALP were decreased, and the expression of IGF-1, ALP, osteocalcin mRNA were reduced in the simulated microgravity group (P＜0.01).Compared with the simulated microgravity group, osteoblasts proliferation rate, IGF-1 , ALP, osteocalcin mRNA were increased in the simulated microgravity + calcitonin group, but no dose-dependent manner could be found. The findings demonstrated that calcitonin can improve osteoblast proliferation and secretion functions via up-regulating IGF-1 mRNA expression in simulated microgravity.关键词
成骨细胞/模拟失重/胰岛素样生长因子1/降钙素/增殖/分泌分类
医药卫生引用本文复制引用
苏嘉霖,张桦,杨理婷,杨锐,王脉桃,黄震..模拟失重状态下成骨细胞胰岛素样生长因子1基因表达与降钙素的干预[J].中国组织工程研究与临床康复,2011,15(15):2672-2676,5.
