浙江医学2011，Vol.33Issue(5)：622-625,4.
急性乙醇中毒对大鼠颅脑损伤后神经系统的影响及其氧自由基损伤机制的研究
Acute ethanol intoxication aggravates traumatic brain injury in rats and its relation to oxygen free radicals
摘要
Abstract
Objective To investigate the influence of acute ethanol intoxication (AEI) on traumatic brain injury (TBI) in rats and its relation to oxygen free radicals.Methods Severe traumatic brain injury model was established by Feeny method in rats, and acute ethanol intoxication was induced by gastric gavage.Forty- eight male SD rats were randomly divided into 4 groups: acute ethanol intoxication group (A), traumatic brain injury group (B), acute ethanol intoxication with traumatic brain injury group (C) and sham operation group (D).The behavioral scores were examined at 1h, 6h, 24h and 72h after injury.After 72h, 6 rats from each group were sacrificed and brain specimens were collected.The apoptotic cells were detected by TUNEL method; the superoxide dismutase (SOD) and malondialdehyde (MDA) were determined.Data were analyzed by one- way ANOVA Results The behavior score of group C was the lowest (P＜0.05) and delayed to recover (P＜0.05).In group C the content of MDA was the highestand SODwas the lowest(P＜0.05); the percentage ofapoptosis cells was the highest among 4 groups (P＜0.05).Conclusion Acute ethanol intoxication exacerbates oxygen free radical damage after traumatic brain injury, increases neural cell apoptosis and affects the recovery of neurological function关键词
急性乙醇中毒/颅脑创伤/超氧化物歧化酶/丙二醛/细胞凋亡Key words
Acute ethanol intoxication / Traumatic brain injury / Superoxide dismutase / Malondialdehyde / Apoptosis引用本文复制引用
汤崇辉,许信龙,傅小君,魏晓捷,潘红松,方战舰..急性乙醇中毒对大鼠颅脑损伤后神经系统的影响及其氧自由基损伤机制的研究[J].浙江医学,2011,33(5):622-625,4.基金项目
宁波市自然科学基金资助项目(2010A610065) （2010A610065）
慈溪市科技局资助项目(CN2008032,CN2009022) （CN2008032,CN2009022）
