中国组织工程研究与临床康复2011，Vol.15Issue(14)：2477-2481,5.DOI:10.3969/j.issn.1673-8225.2011.14.002
地塞米松对大鼠骨髓间充质干细胞凋亡的影响
Effect of dexamethasone on bone marrow mesenchymal stem cells apoptosis in rats
摘要
Abstract
BACKGROUND: Several studies have shown that dexamethasone can selectively promote bone marrow mesenchymal stem cells (BMSCs) apoptosis.OBJECTIVE: To observe the effect of dexamethasone on BMSCs apoptosis and mechanism of action.METHODS: BMSCs (the first generation) were cultured in vitro; BMSCs of passage 2 cultured were divided into 2 groups: control group and experimental group. Dexamethasone was not added into control group, 10-9, 10-8, and 10-7 mol/L concentration of dexamethasone was added into experimental group, respectively. Apoptosis rate was detected by flow cytometer after 3, 6, 12,24 hours. MTT assay was used to detect the effect of dexamethasone on proliferation rate of BMSCs. Effects of BMSCs which were deal with by 10-7 mol/L concentration of dexamethasone after 24 hours on Caspase-3 and bc/-2 were measu red through RT-PCR.RESULTS AND CONCLUSION: Compared with the control group, 10-8, 10-7 mol/L concentration of dexamethasone had significant promotion on the apoptosis of BMSCs after 12-24 hours, which was relative to concentration of dexamethasone and time. With the effect of increasing concentration and prolonged time, the apoptosis rate of BMSCs had an increasing trend. The results of MTT showed that 10-8, 10-7 mol/L concentration of dexamethasone had significant effect on the proliferation rate of BMSCs after 24 hours, which was consistent with the above-mentioned detection results of flow cytometer. RT-PCR indicated that the activity of Caspase-3 was increased significantly and activity of bc/-2 was decreased mildly. The results confirmed that dexamethasone promoted apoptosis of BMSCs through extracellular pathway and (or) mitochondria pathway.关键词
地塞米松/骨髓间充质干细胞/凋亡/Caspase-3/bcl-2分类
基础医学引用本文复制引用
范锲,赵劲民,苏伟,李晓峰..地塞米松对大鼠骨髓间充质干细胞凋亡的影响[J].中国组织工程研究与临床康复,2011,15(14):2477-2481,5.基金项目
国家自然科学基金项目(30860285/H0605) （30860285/H0605）
广西医疗卫生重点科研项目(桂卫重200636). （桂卫重200636）
