中国组织工程研究与临床康复2011，Vol.15Issue(14)：2487-2490,4.DOI:10.3969/j.issn.1673-8225.2011.14.004
大鼠骨髓间充质干细胞成骨分化过程中Hedgehog信号分子的表达
Expression of Hedgehog signaling molecules in the osteogenesis of rat bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND: Hedgehog signaling pathway is an important signal pathway, which regulates the development of variety of tissues and organs in the embryonic stage. It also plays an important role in osteogenesis. However, there are few data about the role of Hedgehog signaling molecules in osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) in vitro.OBJECTIVE: To investigate the expression of Hedgehog signaling molecules during osteogenic differentiation in rat BMSCs in vitro .METHODS: BMSCs were derived from rat bone marrow, cultured and induced by dexamethasone. ldentification of osteoblasts was performed by immunohistochemistry. Level of Sonic hedgehog (SHH) and Indian hedgehog (IHH) during osteogenic differentiation of BMSCs were detected by Western Blot.RESULTS AND CONCLUSION: BMSCs were successfully obtained. Expression of collagen type I was increased on days 7, 14and 21 following dexamethasone induction. In the osteogenic differentiation, a higher expression of SHH protein was detected in osteoblast induced group compared with non-induced group at the same time point (P ＜ 0.05). While IHH expression was decreased and significantly lower in osteoblast induced group compared with non-induced group (P ＜ 0.05). The results indicate that Hedgehog signaling molecules involved in the osteogenic differentiation of BMSCs induced by dexamethasone, and SHH and IHH had difference in osteogenic differentiation of BMSCs.关键词
地塞米松/骨髓间充质干细胞/成骨分化/Hedgehog信号分子/干细胞分类
基础医学引用本文复制引用
张雪萍,马晓丽,汪运山,申红,郏雁飞,赵云,郑燕,肖东杰..大鼠骨髓间充质干细胞成骨分化过程中Hedgehog信号分子的表达[J].中国组织工程研究与临床康复,2011,15(14):2487-2490,4.基金项目
山东省医药卫生发展计划项目(2005JZ001)资助 （2005JZ001）
济南市科技攻关项目(051065). （051065）
