中国组织工程研究与临床康复2011，Vol.15Issue(14)：2530-2534,5.DOI:10.3969/j.issn.1673-8225.2011.14.014
粒细胞集落刺激因子对骨髓极小胚胎样干细胞的动员和募集
Effect of granulocyte-colony stimulating factor on mobilization and recruitment of very small embryonic-like stem cells
摘要
Abstract
BACKGROUND: A population of very small embryonic-like stem cells (VSEL-SCs) is a kind of non-hemopoietic stem cells, which has similar biological characteristics to embryonic stem cells. Currently, most studies concerning VSEL-SCs after myocardial infarction, but mobilization of VSEL-SCs and its effect on repair damaged tissues after acute cerebral infarction remain poorly understood.OBJECTIVE: To investigate the effects of granulocyte-colony stimulating factor (G-CSF) on mobilization of bone marrow-derived VSEL-SCs, recruitment and mechanism after mouse acute cerebral infarction.METHODS: Mouse middle cerebral artery occlusion (MCAO) model was induced by using the filament occlusion method. G-CSF and normal saline were separately injected to MCAO model. Neurological scales were evaluated. The number of VSEL-SCs mobilized into peripheral blood was checked by fluorescence-activated cell sorting analysis. The level of stromal cell derived factor-1 (SDF-1) in plasma and cerebral tissue were determined by ELISA assay. Positive expression of SDF-1 in ischemic regions was detected by immunohistochemistry.RESULTS AND CONCLUSION : Compared with the normal saline group, the neurological behavioral scale of G-CSF treated group was significantly lower at 108 hours after operation (P ＜ 0.05). G-CSF groups mobilized more VSEL-SCs into peripheral blood compared with the operated control group at 72 and 108 hours (P ＜ 0.05). The SDF-1 density significantly increased in the plasma and brain tissues of the G-CSF group than that of the normal saline group (P ＜ 0.05), and positive correlation can be seen between the number of VSEL-SCs mobilized into peripheral blood and the SDF-1 plasma concentration. The positive expressionof SDF-1 in the G-CSF group was greater than that of the normal saline group. The results indicated that G-CSF mobilized VSEL-SCs in adult MCAO mouse bone marrow into peripheral blood. The mechanism of beneficial effects of G-CSF possibly related to increase of SDF-1 expression in the infracted tissues and recruitment of more VSEL-SCs through CXCR4/SDF-1 axis.关键词
极小胚胎样干细胞/骨髓干细胞/神经干细胞/动员/脑梗死/脑修复/小鼠分类
基础医学引用本文复制引用
郭慧娟,王健,张兴秀,郑敏..粒细胞集落刺激因子对骨髓极小胚胎样干细胞的动员和募集[J].中国组织工程研究与临床康复,2011,15(14):2530-2534,5.基金项目
重庆市自然科学基金(2010BB5096),重庆市卫生局科研课题(2009-2-207). （2010BB5096）
