中国组织工程研究与临床康复2011，Vol.15Issue(15)：2821-2824,4.DOI:10.3969/j.issn.1673-8225.2011.15.039
大肠杆菌表达抗原结合片段的策略
Strategies for fragment antigen binding expression in Escherichia coli
摘要
Abstract
BACKGROUND: Low-yields of antigen-binding fragment (Fab) restricts its full scale operation and application, which may be changed by remodeling or optimizing host cells, expression vectors, expression condition and purification conditions.OBJECTIVE: To summarize and introduce new strategies about expression and purification of Fab antibodies in Escherichia coli (E. coli).METHODS: CNKI and Medline databases were searched by the first author using key words of "Escherichia coli, Fab, Expression,antibody, purification" in Chinese or English from year 1990 to 2010. The Fab antibody expression and purification in E. coli were summed up and new researches concerning Fab antibody expression and purification in E. coli were introduced.RESULTS AND CONCLUSION: Totally 125 papers were found and 30 papers were included. The results show that the Fab antibodies are mainly by cytoplasmic expression and secreted expression in E. coli. Its purification methods are mainly by metal ions and chelating affinity purification and specific proteins affinity purification. The influence factors concerning Fab expression in E. coli are correlated to each other. A substantial increase in current research on the latest Fab antibodies in E. coli expression and purification strategies are not consistent optimal, there is no best, only the most suitable.关键词
大肠杆菌/抗原结合片段/表达/纯化/抗体/组织工程分类
医药卫生引用本文复制引用
刘运洪,侯宗柳..大肠杆菌表达抗原结合片段的策略[J].中国组织工程研究与临床康复,2011,15(15):2821-2824,4.基金项目
课题受昆明医学院研究生创新基金项目(2009J10)资助. （2009J10）
