中国组织工程研究与临床康复2011，Vol.15Issue(14)：2577-2582,6.DOI:10.3969/j.issn.1673-8225.2011.14.023
构建携带胞嘧啶脱氨酶基因骨髓间充质干细胞及其对胶质瘤细胞的体外抑制作用
Inhibitory effect of bone marrow mesenchymal stem cells carrying cytosine deaminase gene on glioma cells in vitro
摘要
Abstract
BACKGROUND: The bone marrow mesenchymal stem cells (BMSCs) expressing foreign cytosine deaminase gene (gene CD)can effectively transform 5-fluorouracil (5-FC) that was used before chemotherapy into chemotherapeutics 5-FC with cytotoxicity.BMSCs expressing gene CD significantly inhibited the growth of glioma cells in vitro.OBJECTIVE: To explore the effects of BMSCs as gene therapy vector expressing exogenous gene cytosine deaminase gene on the proliferation of glioma C6 cells.METHODS: Mouse MSCs were isolated and cultured. Lentivirus vector combined with cytosine deaminase gene and green fluorescent protein (GFP) was constructed. Using lentivirus packaging, cytosine deaminase gene and GFP were transferred to mouse BMSCs. BMSCs expressing cytosine deaminase gene and GFP were stably obtained and cocultured with glioma C6 cells.Following 5-FC was added to the medium, effects of cytosine deaminase gene on the proliferation of glioma cells were detected using flow cytometry.RESULTS AND CONCLUSION: Lentivirus-mediated cytosine deaminase gene and GFP gene successfully transfected mouse BMSCs and formed C57BL/6 mMSC-codA/eGFP cells. C57BL/6 mMSC-codA/eGFP could induce obvious apoptosis of glioma C6 cells following treatment with 5-FC. The apoptotic rate of C6 glioma cells was 60％ (P ＜ 0.05) under the action of 5-FC at a concentration of 1×106 μg/L. It was thus concluded that C57BL/6 mMSC-codA/eGFP could convert 5-FC to 5-FU and had significant restriction, even fatal, influence on the growth of C6 glioma cells.关键词
胞嘧啶脱氨酶基因/骨髓间充质干细胞/胶质瘤/慢病毒/载体分类
医药卫生引用本文复制引用
邢琪,宋飞,刘健,姬广春,张大庆,马郁芳..构建携带胞嘧啶脱氨酶基因骨髓间充质干细胞及其对胶质瘤细胞的体外抑制作用[J].中国组织工程研究与临床康复,2011,15(14):2577-2582,6.基金项目
2008年辽宁省教委科研立项(2008Z081)和大连市科学技术基金(2008E13SF203)资助,课题名称:CD/TK单、双自杀基因转染神经干细胞移植治疗脑胶质瘤的研究.2009年辽宁省自然科学基金项目(项目编号:20092165),课题名称:胞嘧啶脱氨酶基因转染骨髓间充质干细胞移植治疗脑胶质瘤的研究. （2008Z081）
