中国组织工程研究与临床康复2011，Vol.15Issue(15)：2730-2734,5.DOI:10.3969/j.issn.1673-8225.2011.15.017
全反式维甲酸诱导小鼠碱性磷酸酶基因启动子区染色体重构
All-trans retinoic acid induces chromatin remodeling at the promoter of mouse alkaline phosphatase
摘要
Abstract
BACKGROUND:Alkaline phosphatase (ALP) is an important biomarker of proliferation and differentiation in osteoblasts. All-trans retinoic acid (ATRA) up-regulates ALP expression in C3H1OT1/2 cells mediated by retinoic acid receptors (RAR) and is irrelevant to the MAPK pathway.OBJECTIVE:To reveal the mechanism of ATRA induced ALP up-regulation from chromosome structure.METHODS:C3H1OT1/2 were treated with ATRA (10-6 mol/L) for 0, 1, 6 and 12 hours, and then DNase Ⅰ hypersensitivity analysis was performed to identify the location of the regulatory regions of ATRA. ChIP assays were performed to analyzed transcription factors bound to regulatory regions of ATRA.RESULTS AND CONCLUSION:DNass Ⅰ hypersensitivity analysis identified the location of regulatory regions of ATRA which exists in the 520 bp region of ALP promoter. ChIP assays proved that ALP up-regulated by ATRA is accompanied by a series of transcription factors binding to ALP promoter. ALP up-regulated by ATRA is accompanied by chromatin remodeling and Histone modification .关键词
碱性磷酸酶启动子/全反式维甲酸/小鼠/C3H10T1/2/染色体重构/组氨酸修饰分类
医药卫生引用本文复制引用
陈迪,孙奋勇..全反式维甲酸诱导小鼠碱性磷酸酶基因启动子区染色体重构[J].中国组织工程研究与临床康复,2011,15(15):2730-2734,5.基金项目
国家自然科学基金项目(81071524)资助. （81071524）
