中国组织工程研究与临床康复2011，Vol.15Issue(15)：2751-2754,4.DOI:10.3969/j.issn.1673-8225.2011.15.022
p38MAPK信号通路与应力介导成肌细胞的凋亡
Role of p38MAPK signaling pathways in the apoptosis of C2C12 myoblast cells subjected to cyclical stretch
摘要
Abstract
BACKGROUND: Because of complicated physiological environment and difficulty to control experimental conditions, it is difficult to get satisfactory results from in vivo studies of cell mechanics.OBJECTIVE: To study the action and mechanism of p38MAPK signaling pathways on myoblast apoptosis based on successful construction of in vitro mechanical stimulation models.METHODS: The C2C12 cells cultured in vitro were divided into control group and SB203580 treatment group. Cyclic tensile stress was applied on the C2C12 myoblast cells for 0, 6, 12 and 24 hours in each group. The Flexcell Strain Unit-5000T was used to expose C2C12 myoblast cell to an equiaxial cyclic of 15% magnitude and a frequency of 10 cycles/min, each cycle including the 3 s stretch and 3 s relaxation. Hoechst 33258 fluorescent staining and optical microscope were used to detect cell apoptosis. RT-PCR, flow cytometric analysis were used to observe the apoptosis of C2C12 myoblast cells and Western blotting were used to detect the activity of p38MAPK and p-p38MAPK. RESULTS AND CONCLUSION: The optical microscope tested the change in the morphology. Hoechst 33258 staining showed that after treatment with cyclic stress, the cell took the typical appearance of apoptosis with chromatin condensation and apoptotic bodies. RT-PCR and flow cytometry showed that with the extension of time the rate of the apoptosis of C2C12 myoblast cell increased. And cells imposed SB203580 before imposing cyclical tensile stress, the results showed that the apoptosis was markedly affected, and the p-p38MAPK expression declined apparently. These findings demonstrate that p38MAPK signaling pathways in stress mediated into C2C12 myoblast cell apoptosis plays an important role.关键词
细胞凋亡/C2C12/p38MAPK/周期性张应力/成肌细胞分类
基础医学引用本文复制引用
田臻,刘文,杨竹丽,贾文敏,袁晓,仇静,达雨,杜衍晓,于江波,张月..p38MAPK信号通路与应力介导成肌细胞的凋亡[J].中国组织工程研究与临床康复,2011,15(15):2751-2754,4.基金项目
国家自然科学基金资助项目(30871426).the National Natural Science Foundation of China,No.30871426 （30871426）
