中国农业大学学报2011，Vol.16Issue(1)：73-78,6.
荷斯坦阉牛瘤胃液中阿魏酸酯酶和乙酰酯酶的酶学特性研究
Enzymological characterization of feruloyl and acetyl esterases in the rumen fluids of Holstein steers
摘要
Abstract
To determine enzymological characteristics of feruloyl esterase (FAE) and acetyl esterase (AE), rumen fluids collected from Holstein steers intalled flexible ruminal cannula were centrifuged at 1 000g at 4 ℃ for 10 min to prepare crude enzyme solutions.The optimal pH is 9.0 and optimal temperature for FAE is 40 - 50 ℃ ;and the Michaelis constants (Km) is 0.76 mmol/L and maximum velocities (Vmax) against methyl ferulate is 5.43 mU at pH 6.0 and 39 ℃.The optimal pH for AE is 8.0 and optimal temperature for AE is 50 ℃ ,and the Km and Vmax against p-nitrophenyl acetate at pH 7.0 and 39 ℃ were 0.64 m mol/L and 91.00 mU.Cations such as Mg2 + , Zn2 + , K + ,Cu2 + , Co2 + ,Ca2 + and Mr2+ in this study inhibited the activities of esterases for FAE while Fe2+ has opsite effects.The cations of Mg2+ , K+ ,Co2 + ,Ca2 + and Mr2 + had a stimulatory effect on AE.In general, FAE showed relatively more stable than AE in the rumen though both pH and temperature in rumen fluids may not be optimum for both esterases to exert their enzymological potentials in plant cell wall degradation.关键词
牛/瘤胃液/阿魏酸酯酶/乙酰酯酶/酶学特性分类
农业科技引用本文复制引用
杨红建,岳群,曹阳春..荷斯坦阉牛瘤胃液中阿魏酸酯酶和乙酰酯酶的酶学特性研究[J].中国农业大学学报,2011,16(1):73-78,6.基金项目
国家自然科学基金资助项目(31072054) （31072054）
国家现代肉牛产业技术体系建设任务(nycytx-38) （nycytx-38）
