中国神经再生研究（英文版）2011，Vol.6Issue(10)：738-743,6.DOI:10.3969/j.issn.1673-5374.2011.10.003
A novel ethanol-based method to induce differentiation of adipose-derived stromal cells into astrocytes
A novel ethanol-based method to induce differentiation of adipose-derived stromal cells into astrocytes
Ya Ou 1Xiaodong Yuan 1Yanan Cai 1Yanhui Lu1
作者信息
摘要
Abstract
The quantity and survival time of astrocytes, which were differentiated from adult adipose-derived stromal cells after exposure to an inducer containing 3-isobutyl-1-methylxanthine, have thus far been unsatisfactory. The present study investigated the growth and differentiation characteristics of induced astrocytes by observing their growth curves. After induction for 48 hours with an inducer containing 0.5% ethanol, some adult adult adipose-derived stromal cells displayed typical astrocytic morphology. The cell quantity gradually decreased with prolonged induction time. Nestin, glial fibrillary acidic protein, and S-100 expression reached peak levels at 14 days, but neuron-specific enolase was not expressed. These results suggest that the induced astrocytes reached their peak at 14 days. Further optimization of the culture environment may yield mature astrocytes with normal functions, in greater quantity, and prolonged survival time.关键词
adult adipose-derived stromal cells/induced differentiation/astrocyte/morphology/growth characteristics/neural regenerationKey words
adult adipose-derived stromal cells/induced differentiation/astrocyte/morphology/growth characteristics/neural regeneration引用本文复制引用
Ya Ou,Xiaodong Yuan,Yanan Cai,Yanhui Lu..A novel ethanol-based method to induce differentiation of adipose-derived stromal cells into astrocytes[J].中国神经再生研究（英文版）,2011,6(10):738-743,6.
