中国农业科技导报2011，Vol.13Issue(1)：122-128,7.DOI:10.3969/j.issn.1008-0864.2011.01.20
基于分子印迹敏感膜和白光反射干涉的残留药物检测技术
Drug Residue Detecting Technology Based on Combing Molecular Imprinted Polymer Film with Reflectometric Interference Spectroscopy
摘要
Abstract
To measure optical thickness of a molecularly imprinted polymer film whose surface was not smooth with porous structure, a ladder model for RIfS (refiectometric interference spectroscopy) was established, and a computer program for full-spectrum fitting of RIfS spectra was written based on damped least square method. Then the optical thickness of a monolayer or bilayer film could be obtained by fitting the experimental spectrum data. Sensitive films were fabricated by spin coating the mixture of CAP (chloramphenicol) imprinted nano-spheres and polystyrene (PS) onto glasses and silicon wafers, respectively. Linear relationships between variance of film optical thickness and concentrations of CAP in solution were founded. The detection limits were 0. 229 1 mmol/L and 0. 185 2 mmol/L, respectively. Then a real sample ( 1 g milk fortified with 0. 2 mL 0.5 mol/M CAP) was analyzed by this method. After diluting the extracted liquid and combing the standard curve, the CAP recovery was 91％, which agreed with that determined by HPLC. This result indicated that the combination of molecularly imprinted polymer films and RIfS was feasible in analyzing drugs residue.关键词
光学厚度/分子印迹纳米微球/敏感膜/旋涂法/白光干涉分类
化学化工引用本文复制引用
刘秋明,王永向,丁艳,戎非,付德刚..基于分子印迹敏感膜和白光反射干涉的残留药物检测技术[J].中国农业科技导报,2011,13(1):122-128,7.基金项目
国家863计划项目(2006AA10Z436)资助. （2006AA10Z436）
