中国真菌学杂志2011，Vol.6Issue(1)：10-14,5.
新疆地区白念珠菌基因型分析及其体外药物敏感性研究
Genotype analysis and antifungal susceptibility of Candida albicans in Xinjiang area
摘要
Abstract
Object To analyze the genotypes of 50 isolates of Candida albicans from Han and Uyghur patients in Xinjiang area and to understand the in vitro antifungal susceptibilities of these isolates against amphotericin B, flucytosine, micafungin, itraconazole, fluconazole and miconazole. Methods The genotypes were identifed by the size of PCR amplicons amplifined from the group Ⅰ intron contained region of 25S rDNA ofC. albicans :genotype A (450 bp) ,genotype B (840 bp) and genotype C (450 bp and 840 bp). In vitro antifungal susceptibilities of 50 strains to 6 kind of antifungal agents were determined by CLSI M27-A microdilution method. Results Fifty strains were classified into three genotypes,including 30 strains of type A and 10 strains of type B and C,all these strains showed lower MIC to amphotericin B,flucytosine,micafungi ,and miconazole ( MIC range:0.25-0.5 μg/mL,0. 125-0.25 μg/mL, ≤0.03 μg/mL, and 0.25-8 μg/mL, respectively), and higher MIC to itraconazole and fluconazole ( MIC range :0.25-8 μg/ mL and 0.5-64 μg/mL,respectively). The MIC of the genotype B and C strains to flucytosine was 0. 125 μg/mL. The resistant ratio of itraconazole and fluconazole were 84％ and 70％ , respectively. No statistical significance was found in the genotypes of the strains from different races ( P ＞ 0. 05 ) or different genotypic strains and antigungal susceptibilities ( P ＞ 0. 05 ). Conclusions C. albicans in Xinjiang area included 3 genotypes ( A, B and C). There was no correlation between the genotype and antifungal susceptibilities of C. albicans strains from Han and Uyghur patients. Nither did genotype and race. All strains were resistant to itraconazole and flucytosine, but genotype B and C showed high susceptibilities to flucytosine.关键词
新疆地区/白念珠菌/基因型/体外药敏分类
医药卫生引用本文复制引用
帕丽达·阿布利孜,哈木拉提·吾甫尔,Takashi Yaguchi,Kayoko Takizawa,李若瑜..新疆地区白念珠菌基因型分析及其体外药物敏感性研究[J].中国真菌学杂志,2011,6(1):10-14,5.基金项目
国家自然科学基金(30960351) （30960351）
新疆少数民族科技人才特殊培养计划科研项目(200823113) （200823113）
日本五峰生命科学基金(18405005). （18405005）
