中国医科大学学报2011，Vol.40Issue(1)：27-29,3.
JCV病毒T抗原诱发晶状体上皮肿瘤动物模型建立
The Establishment of Transgenic Mouse Model of Lens Epithelial Tumors Induced by JC Virus T Antigen
摘要
Abstract
Objective To establish the transgenic mouse model of spontaneous lens epithelial tumors using JCV T antigen initiated by α-crystallin promoter. Methods JCV T antigen was separated from pBSK (pBluescript SK)-T antigen plasmid by restriction enzyme BclI and compatibly ligated downstream α-crystallin promoter via BamH I. DNA fragments containing T antigen and α-crystallin promoter were separated from constructant by NciI and then microinjected into the fertilized eggs of C57BL/6J mice. The positive mice were screened by PCR and the comparative copies of T antigen were determined by real-time PCR. The lens of transgenic mice was grossly and histologically examined.The T antigen, p53 and β-catenin were examined in the lens lesions by immunohistochemistry. Results The α-crystallin-JCV T antigen expressing plasmid was successfully constructed. The T antigen copies of the No. 2(αAT-2 )transgenic mouse were highest in the 5 transgenic mice and displayed tumor-like lesion with the strong expression of T antigen, p53 and β-catenin proteins. Conclusions The transgenic tumor model provides a direct evidence for the oncogenic role of JCV T antigen insertation in the epithelial cells, a good animal model to investigate the oncogenic mechanisms of JCV T antigen, screen the anti-tumor reagents and monitor the gene therapy.关键词
JC病毒/T抗原/转基因鼠/晶状体分类
医药卫生引用本文复制引用
王建平,张梅英,徐小燕,王巍,夏蒲,郑志宏,王禄增,高野康雄,郑华川..JCV病毒T抗原诱发晶状体上皮肿瘤动物模型建立[J].中国医科大学学报,2011,40(1):27-29,3.基金项目
辽宁省百千万人才工程资助项目(2008921066) （2008921066）
教育部归国人员科研启动基金(2009-03) （2009-03）
人事部留学人员科技活动择优资助项目(2008-01) （2008-01）
2009年沈阳市科学技术计划资助项目(1091175-1-00) （1091175-1-00）
沈阳人才专项基金(2009-01) （2009-01）
