中国肿瘤生物治疗杂志2011，Vol.18Issue(1)：28-32,5.DOI:10.3872/j.issn.1007-385X.2011.01.006
消癌解毒方抑制肝癌H22移植瘤的生长及其机制
Xiaoai Jiedu Recipe inhibits transplanted hepatocarcinoma H22 tumors and the related mechanism
摘要
Abstract
Objective: To study the inhibitory effects of Xiaoai Jiedu Recipe(XJR) against transplanted hepatocarcinoma H22 tumors in mice and the related mechanism. Methods: H22-transplanted tumor mouse models were established and were divided into control group, XJR treatment groups ( 10, 30, 90 g/kg), and cisplatin treatment group. The growth of tumors in different groups was measured, and pathology changes of transplanted tumor tissues in different groups were examined by H-E staining. Flow cytometry was applied to examine cell cycle and sub-diploid apoptosis rate of transplanted tumor cells, and VEGF levels in the peripheral blood of mice were measured by ELISA. Results: Compared with control group, different XJR groups and the cisplatin group significantly inhibited growth of H22-transplanted tumors (24. 5％,42.8％, 21.1,58.6％ vs 0, P ＜0.05, P ＜0.01 ), cells in XJR groups showing massive necrosis and obvious chromosome condensation. Medium-dosage XJR and cisplatin blocked transplanted tumor cells in G0/G1 phase ( P ＜ 0. 05 ), and the cells in S phase were significantly reduced ( P ＜ 0. 05 ). The sub-diploid apoptosis rate of transplanted tumor cells in medium-doseage X JR group was similar to that in cisplatin group (60.52 ± 6.40 vs 71.32 ± 16.02, P ＞ 0. 05 ). Peripheral VEGF levels were significantly lower in the medium-, large-dosage XJR groups and cisplatin group than in the control group ( 104.3 ± 6.1, 105.8 ± 7.2, 88.6 ± 4.3 vs 120.7 ± 12.6, P ＜ 0.05, P ＜ 0.01 ). Conclusion: Xiaoai Jiedu Recipe can inhibit the growth and promote apoptosis of H22-transplanted tumor cells, and inhibition of VEGF may be one of the mechanisms for the anticancer effect.关键词
消癌解毒方/H22肝癌细胞/移植瘤/血管内皮细胞生长因子(VEGF)/增殖/凋亡分类
医药卫生引用本文复制引用
陈海彬,沈波,李黎,程海波,周红光,王明艳,吴勉华..消癌解毒方抑制肝癌H22移植瘤的生长及其机制[J].中国肿瘤生物治疗杂志,2011,18(1):28-32,5.基金项目
国家自然科学基金资助项目(No.30973742/C190201) （No.30973742/C190201）
江苏省自然科学基金资助项目(No.BK2007240) （No.BK2007240）
江苏省高校自然科学研究面上项目资助(No.09KJB360003) （No.09KJB360003）
江苏省普通高校研究生科研创新项目资助(No.CX09S_037Z) （No.CX09S_037Z）
江苏省中医药局课题(No.LB09032). （No.LB09032）
