中药材2011，Vol.34Issue(1)：4-8,5.
氨磺灵离体诱导半枝莲多倍体的研究
Oryzalin-induced Chromosome Doubling of Scutellaria barbata
岳敏 1陈崇顺 1廖静 1孙晶 1沈唯军1
作者信息
- 1. 南京师范大学生命科学学院/江苏省生物多样性与生物技术重点实验室,江苏,南京,210046
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摘要
Abstract
Objective:To obtain polyploidy Scutellaria barbata. Methods:Explants of stem segment of Scutellaria barbata were treated with oryzalin at various concentration and time duration in vitro. Results:Comparing with the characteristics of diploid, mutant plants had significant changes in plant morphology inducing compact plant, darker leaf color, smaller leaf index, thicken stem, significantly larger stomata,guard cells and chloroplasts in stomata guard cells and significantly lower stomata density. Mutant plants were plant chimera of diploid and tetraploid assessed by chromosome counting and flow cytometry. Conclusion:The technique could be used for chromosome doubling in Scutellaria barbata.关键词
半枝莲/氨磺灵/多倍体/诱导/鉴定分类
医药卫生引用本文复制引用
岳敏,陈崇顺,廖静,孙晶,沈唯军..氨磺灵离体诱导半枝莲多倍体的研究[J].中药材,2011,34(1):4-8,5.
