中国畜牧兽医2011，Vol.38Issue(1)：47-50,4.
奶牛结核分枝杆菌rpoB和katG基因突变与多重耐药的相关性
Cows Mycobacterium tuberculosis rpoB and katG Gene Mutations Associated with Multi-drug Resistance
摘要
Abstract
The study of cows Mycobacterium tuberculosis rpoB and katG gene mutations and the relation between drug resistance.Take 30 strains of clinical isolates of M.tuberculosis from cattle, use of phage amplified biologically assay on the drug sensitivity of Rifampicin and isoniazid, polymerase chain reaction-DNA direct sequencing rpoB and katG gene were amplified and sequenced, analysis of rpoB and katG gene mutations and drug resistance associate.The results showed that 15 resistant strains in four were resistant to RFP, three of them found mutations at condon 531 occurred in 75.00％ (3/4); 9 single INHresistant strains of seven mutations,6 isolates mutation at condon 315 occurred in 85.71％ (6/7), 1 isolate mutation at condon 463 occurred in 14.29％(1/7); 2 isolates were resistant to RFP and INH, sequencing showed that two were both mutations at condon 531 in the rpoB gene and condon 315 in the katG gene.All strains were not found the deletion of rpoB and katG gene.The main mechanism of RFP and INH drug resistance is mutations at condon 531 in the rpoB gene and condon 315 in the katG gene; while detection of bovine tuberculosis RFP-resistance, it can be initial screening MDR M.tuberculosis.关键词
结核分枝杆菌/利福平/异烟肼/rpoB基因/katG基因/多重耐药分类
农业科技引用本文复制引用
赵莉,纳玮,周学章,王玉炯..奶牛结核分枝杆菌rpoB和katG基因突变与多重耐药的相关性[J].中国畜牧兽医,2011,38(1):47-50,4.基金项目
国家重点基础研究发展计划"973"项目(2006CB504400) （2006CB504400）
高等学校科技创新工程重大项目(706057). （706057）
