中国畜牧兽医2011，Vol.38Issue(1)：81-88,8.
猪脑心肌炎病毒GXLC株的分离及其3D基因分子特征的分析
Isolation of Porcine Encephalomyocarditis Virus GXLC Strain and Analysis of the Molecular Characteristics of its 3D Gene
摘要
Abstract
The clinical tissue samples collected from piglets died of suspected encephalomyocarditis were homogenated and the supernatant was inoculated into baby hamster kidney (BHK-21) cell lines to isolate encephalomyocarditis virus (EMCV).According to the cytopathic effect (CPE) and the results of RT-PCR and indirect fluorescent antibody test (IFA) ,one strain of EMCV was successfully isolated and designated as GXLC strain. The 3D gene of GXLC strain was amplified by RT-PCR,cloned into pMD18-T vector, sequenced and analyzed. The sequence analysis showed that the 3D gene of GXLC strain consisted of 1380 nucleotides encoding 460 amino acids and contained 7 epitopes. The homology analysis indicated that the homology of 3D gene of GXLC strain and other EMCV strains available in GenBank were from 84.7％ to 99.7％ and from 96.1％ to 99.6％ based on the nucleotide and deduced amino acide sequences, respectively. The phylogenetic tree based on the 3D gene nucleotide sequences revealed that all the EMCV strains could be divided into two groups: group Ⅰ and group Ⅱ ,and group Ⅰ could be subdivided into subgroup Ⅰ a and subgroup Ⅰ b. The strains from pig distributed in group Ⅰ and group Ⅱ ,while the strains from mouse distributed in group Ⅰ and the strains from human distributed in group Ⅱ . GXLC strain and other EMCV isolates from China belonged to subgroup Ⅰ a.关键词
猪/脑心肌炎病毒/分离/3D基因/分子特征分类
生物科学引用本文复制引用
陈进喜,施开创,黄胜斌,陆文俊,屈素洁,郑敏,李军..猪脑心肌炎病毒GXLC株的分离及其3D基因分子特征的分析[J].中国畜牧兽医,2011,38(1):81-88,8.基金项目
广西科学基金项目(桂科青0728047) （桂科青0728047）
广西科技创新能力与条件建设基金项目(08-05-01D). （08-05-01D）
