中国畜牧兽医2011，Vol.38Issue(1)：100-104,5.
肝脏特异性表达载体的构建及Western blotting检测
The Construction and Western Blotting Ananlysis of Liver-specific siRNA
摘要
Abstract
In order to achieve tissue-specific siRNA therapy, the liver-specific siRNA in vivo expression plasmid had been investigated. It could be used for liver-specific hepatitis B treatment,overcoming a barrier about the siRNA in the clinical application. We used anti-EGFP siRNA instead of the anti-HBV siRNA in this study. The vector was expressed specifically in hepatocyte through construction can express siRNA. The constructed vector had been transiently transfected HepG2,MDA-MB-231,MDA-MB-293 cells respectively. Then the inhibition of siEGFP in the different cell lines had been evaluated and compared by Western blotting ananlysis. siEGFP was proved by Western blotting result to be more efficient to inhibit in the hepatic tissue origin clone HepG2 than other two groups. Tissue specificity was achieved through constructed carrier expressing siRNA specifically in hepatoma cell line, but not in other tissue cell. If we replaced the siEGFP with anti-HBV siRNA in this modified plasmid,it was highly possible to develop a liver-specifically expressing anti-HBV siRNA therapy.关键词
RNAi/组织特异性siRNA/肝脏特异性/EGFP/Western blotting分类
生物科学引用本文复制引用
张利涛,刘慧琳,曹以诚..肝脏特异性表达载体的构建及Western blotting检测[J].中国畜牧兽医,2011,38(1):100-104,5.基金项目
国家自然科学基金重大研究计划项目(90412015). （90412015）
