中国畜牧兽医2011，Vol.38Issue(1)：112-115,4.
贵州地区牛源金黄色葡萄球菌分离株16S rRNA分子鉴定及RAPD分型研究
Research of Identification by 1 6S rRNA and RAPD Typing on Staphylococcus aureus Isolated from Dairy Cattle in Guizhou
摘要
Abstract
The 16S rRNA conserved sequence was used for isolation and identification of Staphylococcus aureus (S. aureus)strains causing bovine mastitis in Guizhou,and system of randomly amplified polymorphic DNA (RAPD) was established for research on genetic polymorphism of the S. aureus strains. The results showed that 37 S. aureus strains were isolated and identified from 69 mastitis milk samples. The 37 S. aureus strains amplified out 1 to 7 electrophoresis strips with sizes ranging from 400 to 4500 bp by RAPD. The strain were separated to 7 genotypes according to the fingerprint, 7 strain were type Ⅰ (18.9 ％), 2 strains were type Ⅱ (5.4 ％ ), 2 strains were type Ⅲ (5.4 ％ ), 1 strains was type Ⅳ (2.7 ％), 19 strains were type Ⅴ (51.4％) ,1 strain was type Ⅵ (2.7％) and 5 strains were typeⅦ (13.5％). The preponderant S. aureus strains in this area was typeⅤ. Effects of geography and climate on transmission of pathogenic bacteria was the mainly reason of discrepancy on proportion of different genotype strains.关键词
奶牛乳房炎/金黄色葡萄球菌/16S rRNA/RAPD/基因型分类
农业科技引用本文复制引用
邓海平,蒲万霞,梁剑平,倪春霞,孟晓琴..贵州地区牛源金黄色葡萄球菌分离株16S rRNA分子鉴定及RAPD分型研究[J].中国畜牧兽医,2011,38(1):112-115,4.基金项目
国家科技支持计划(2006BAD04A05). （2006BAD04A05）
