利用VP3蛋白初步建立鸡传染性法氏囊病间接ELISA方法OA北大核心CSTPCD
Preliminary Establishment of Indirect ELISA Based on the VP3 Structural Protein of IBDV
本研究对从河北某鸡场分离的IBDV分离株的VP3基因进行了克隆和表达,将表达蛋白用亲和层析的方法进行纯化,纯化后的蛋白包被ELISA反应板,初步建立了一种用于检测IBDV抗体的间接ELISA方法.
In this study, VP3 gene was cloned and expressed from IBDV field isolate, which was isolated from a poultry farm in Hebei province.After purification using affinity chromatography, the fusion protein was used as a coating antigen for the preliminary establishment of an indirect ELISA for IBDV antibody detection.
宋铁彬;陈广东
朝阳出入境检验检疫局,辽宁朝阳,122000朝阳出入境检验检疫局,辽宁朝阳,122000
畜牧业
鸡传染性法氏囊病病毒VP3蛋白间接ELISA
《中国畜牧兽医》 2011 (1)
116-118,3
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