中国畜牧兽医2011，Vol.38Issue(1)：198-201,4.
猪繁殖与呼吸综合征病毒变异野毒株与JXA1-R疫苗毒株快速鉴别方法的建立
Establishment of Distinguishing Method on Detecting Porcine Reproductive and Respiratory Syndrome Virus JXA1 and JXA1-R Strain
何玲 1裴仉福1
作者信息
- 1. 广东大华农动物保健品股份有限公司,广东新兴,527400
- 折叠
摘要
Abstract
Based on the variant sequences of PRRSV JXA1 strain and the genetic markers of JXA1-R strain, two pairs of primers were designed and synthesized,and RT-PCR methods were established for detecting the two viruses with specific gene fragments of 400 bp and 290 bp.There was no cross-reactions with those clinically relevant pathogens,such as classical swine fever virus, porcine pseudorabies virus, porcine circovirus type 2, porcine parvovirus.The sensitivity tests showed that the assay could detect 103 copies/μL RNA of JXA1 and 104 copies/μL RNA of JXA1-R.The RT-PCR established in this study, with high specificity and sensitivity,was very suit for clinic-sample detection.关键词
PRRSV变异株/JXA1-R/RT-PCR分类
农业科技引用本文复制引用
何玲,裴仉福..猪繁殖与呼吸综合征病毒变异野毒株与JXA1-R疫苗毒株快速鉴别方法的建立[J].中国畜牧兽医,2011,38(1):198-201,4.
