作物学报2011，Vol.37Issue(1)：87-94,8.DOI:10.3724/SP.J.1006.2011.00087
ET-ISJ标记的开发及陆地棉遗传图谱构建
Development of Exon-Targeted Intron-Exon Splicing Conjunction (ET-ISJ) Marker and Establishment of Upland Cotton Genetic Map
摘要
Abstract
Cotton is the leading fiber crop in the world, and upland cotton contributes over 95％ of cotton production.The genetic map of upland cotton is far from saturated, so it is necessary to develop new markers for genetic map construction.ET-ISJ (exon targeted intron-exon splice conjunctions) marker primers were designed according to the conserved intron-exon splicing junction sequences.A total of 1280 ET-ISJ primer combinations were used to screen polymorphism between upland cotton cultivar Yumian1 and T586, and 69 of which showed polymorphism, accounting for 5.4％ of total primer combinations.The 69 polymorphic ET-ISJ primer combinations were used to genotype 270 F2:7 recombinant inbred line derived from (Yumian 1 × T586), and 70 ET-ISJ loci were obtained.Linkage analysis was conducted on 70 ET-ISJ, 523 SSR, 59 IT-ISJ, 29 SRAP and 8 morphological loci, and a linkage map including 59 linkage groups and 673 loci (68 ET-ISJ, 510 SSR, 58 IT-ISJ, 29 SRAP and 8 morphological loci) was established.The linkage map covered 3 216.7 cM, accounting for 72.3％ of cotton genome, with an average interval of 4.8 cM between two markers.Sixty-eight ET-ISJ loci were located on 20 chromosomes.The present study demonstrated that ET-ISJ markers were stable, highly polymorphic, and able to be effectively applied in genetic map construction of cotton and other plant’s.关键词
陆地棉/外显子定靶内含子-外显子剪接位点标记/遗传图谱引用本文复制引用
林刚,张轲,张建,滕中华,张正圣..ET-ISJ标记的开发及陆地棉遗传图谱构建[J].作物学报,2011,37(1):87-94,8.基金项目
本研究由国家自然科学基金项目(30871556,30900910)和国家高技术研究发展计划(863计划)项目(2006AA10Z1D3)资助. （30871556,30900910）
