中国油料作物学报2011，Vol.33Issue(1)：39-43,5.
新型植物油莎豆DNA提取与SRAP体系优化
DNA extraction and establishment of SRAP reaction system in tigernut (Cyperus esculentus)
摘要
Abstract
In this study, fourteen tigernut lines from different geographical areas were used to investigate the effective method of DNA extraction and to construct the optimized SRAP reaction system.Twenty seven reaction systems were designed with three concentration levels of DNA template, magnesium ion and primers.The results showed that the improved method of CTAB contributed to a better extraction of genomic DNA of tigernut, and the A260/280 values of all samples ranged from 1.70 to 1.98.Out of the 27 reaction systems, the optimal system for SRAP analysis had 25ng DNA, 1.5mmol/L MgCl2, 1μmol/L primers, 0.3mmol/L dNTPs and 1U Taq polymerase in 15μL total volume.These results provided technical assistance to evaluate germplasm resource, genetic diversity and molecular breeding in tigernut.关键词
油莎豆/DNA提取/SRAP/体系优化分类
农业科技引用本文复制引用
赵永国,金梦阳,危文亮..新型植物油莎豆DNA提取与SRAP体系优化[J].中国油料作物学报,2011,33(1):39-43,5.基金项目
国家自然科学基金(31000727) （31000727）
湖北省自然科学基金(2008CDB092) （2008CDB092）
农业部油料作物生物学重点开放实验室开放基金(2010-1) （2010-1）
