中国药理学通报2011，Vol.27Issue(5)：632-638,7.DOI:10.3969/j.issn.1001-1978.2011.05.010
塞来昔布联合多柔比星对乳腺癌细胞增殖及凋亡的影响
Effects of celecoxib combined with doxorubicin on the proliferation and apoptosis of breast cancer cells
摘要
Abstract
Aim To investigate the anti-tumor effects of COX-2 selective inhibitor celecoxib combined with doxorubicin on breast cancer MCF-7 cells and Sk-Br-3 cells and to discuss whether celecoxib has the functions of attenuating doxorubicin's toxicity and enhancing its efficiency. Methods Cells were divided into control group, celecoxib group, doxorubicin group and combined group. MTT assay was used to detect growth inhibition induced by drugs in MCF-7 cells and Sk-Br-3 cells. Western blot were used to determine the expression of bcl-2 in MCF-7 cells and Sk-Br-3 cells. PI staining detected cell apoptosis. Clone forming determined drug's cytotoxicity in MCF-7 cells and Sk-Br-3 cells.Results MTT results showed that 72 h after the action of 30 μmol · L-1 celecoxib combined with 0. 625 mg ·L-1 doxorubicin in MCF-7 cells. the survival rate was 68. 53％ , in Sk-Br-3 cells, the survival rate was 32. 66％ . The survival rate of breast cancer cells was significantly lower than that of doxorubicin group. Celecoxib combined with doxorubicin showed time and dose-dependent effect. PI staining by flow cytometry showed that celecoxib combined with doxorubicin induced apoptosis rate of MCF-7 cells was 59. 7％ , and that of Sk-Br-3 cells was 65. 8％ , which significantly increased apoptosis rate compared with doxorubicin alone ( MCF-7 cells 25. 9％ , Sk-Br-3 cells 28. 7％ ).Western blot showed that doxorubicin alone could reduce the expression of bcl-2 and the combined group reduced more than the single ones. Activity of caspase3 was increased by celecoxib combined with doxorubicin than that treated by doxorubicin alone.Conclusion Celecoxib and doxorubicin show proliferation inhibition and apoptosis promotion effects on breast cancer MCF-7 cells and Sk-Br-3 cells. The combination of the two shows synergistic or additive effect.关键词
乳腺癌/塞来昔布/多柔比星/细胞增殖/凋亡/协同作用分类
医药卫生引用本文复制引用
苏方,刘浩,程秀,方琳,宋乐乐,马琳艳,陈超,蒋志文..塞来昔布联合多柔比星对乳腺癌细胞增殖及凋亡的影响[J].中国药理学通报,2011,27(5):632-638,7.基金项目
国家自然科学基金资助项目(No 81000992,81072207) （No 81000992,81072207）
安徽省自然科学基金项目(No 090413135) （No 090413135）
安徽省高等学校优秀青年人才基金项目(No 2009SQRZ134ZD) （No 2009SQRZ134ZD）
