中国组织工程研究与临床康复2011，Vol.15Issue(10)：1794-1798,5.DOI:10.3969/j.issn.1673-8225.2011.10.018
鼻腔内长期输送脐血单个核细胞对大鼠脑梗死体积及行为学的影响
Effects of long term intranasal delivering umbilical cord blood mononuclear cells on cerebral infarct volume and behaviors in rats
摘要
Abstract
BACKGROUND: It was a new non-invasive method for intranasal delivering cell or cytokine into the brain, which could overcome the peripheral side-effect of other delivering methods.OBJECTIVE: To explore the effect of long term intranasal delivering human umbilical cord blood mononuclear cells (UCBMCs) on changes in behaviors and infarct volume of brain infarct in rats.METHODS: Middle cerebral artery of Wistar rat was directly cut to establish models of cerebral infarction. Rat models with equal neurological score were randomly assigned to control and mononuclear cell groups. In the mononuclear cell group, UCBMCs of the third generation were intranasally injected. In the control group, phosphate buffer saline was infused, once a day, totally 6 weeks. Behavior was assessed once a week. Brain infarct volumes were quantified following the last behavior assessment.RESULTS AND CONCLUSION : Compared with control group, modified neurological severity score was improved in the mononuclear cell group at 3 weeks after treatment, and lasted at 5 weeks (P ＜ 0.05, P ＜ 0.01). In the mononuclear cell group, the mean latency in Morris Water Maze Test shortened significantly at 1-5 weeks following treatment (P ＜ 0.05, P ＜ 0.01), and the effect was obvious over time. Identical to behavior improvement, the cerebral infarct volume in the mononuclear cell group (9.1 5±4.36)％ was smaller compared with (30.56±4.65)％ (P ＜ 0.01). Early and long term of intranasal delivering UCBMCs to treat brain infarct model rats could improve their behavior score and shrink their infarct volume, which could be considered as a simple and feasible method to treat brain infarct.关键词
脑梗死/脐血/单个核细胞/大鼠/干细胞分类
基础医学引用本文复制引用
曾宪智,沈慧娟,王洋,朱群娥,郭连军,叶先才..鼻腔内长期输送脐血单个核细胞对大鼠脑梗死体积及行为学的影响[J].中国组织工程研究与临床康复,2011,15(10):1794-1798,5.基金项目
浙江省嘉兴市科技计划项目(2010AY1097) （2010AY1097）
浙江省大学生科技创新项目(2010R417021). （2010R417021）
