中国组织工程研究与临床康复2011，Vol.15Issue(13)：2299-2303,5.DOI:10.3969/j.issn.1673-8225.2011.13.006
假体置入患者外周血白细胞Toll样受体2和4的表达
Toll-like receptor 2 and 4 expression in peripheral blood leukocytes of patients following prosthesis implantation
摘要
Abstract
BACKGROUND: Toll-like receptors (TLRs) are expressed on the surface of biofilms in loosening hip prosthesis. However, the expression changes remain unclear.OBJECTIVE: To investigate the expression of TLR 2 and 4 in leukocytes of patients undergoing joint prosthesis implantation.METHODS: Fasting blood plasma samples were harvested from elbow veins 24 hours post admission and 3 days post-operation from patients undergoing arthroplasty or arthroscopy (n=l0). Cell-associated TLR 2 and 4 was measured by flow cytometry, white blood cells, C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were tested.RESULTS AND CONCLUSION: WBC, ESR, CRP were at normal level in both groups. TLR2 and 4 were mostly expressed in monocytes of peripheral blood, with low ex pression levels in lymphocytes and neutrophils. The expression levels of TLR2 was significantly reduced following operation (P＜0.05), but no significant difference was observed in the expression levels of TLR4 in arthroplasty group or TLR2 and 4 in arthroscopy group between post-operation and pre-operation (P＞0.05), whereas a significant change of TLR9 expression gap was found in arthroplasty patients before and after the procedure (P＜0.05). TLR 4expression remained unchanged (P＞0.05). The TLR2 positive expression rate in monocytes of peripheral blood was significantly lower in arthroplasty group compared with arthroscopy group (P＜0.05), but no difference was found in TLR4 expression. Results show that in the absence of clinical infection, stress and tissue damage during arthroplasty and arthroscopy do not affect TLR2and 4 expression, but TLR2 expression was downregulated following implantation of joint prosthesis.关键词
关节置换/假体/Toll样受体/流式细胞术/人工假体分类
医药卫生引用本文复制引用
张紫机,康焱,翟齐毅,张浩,张阳春,侯昌禾,李子卿,盛璞义,廖威明..假体置入患者外周血白细胞Toll样受体2和4的表达[J].中国组织工程研究与临床康复,2011,15(13):2299-2303,5.基金项目
广东省科委国际合作项目(2006B50107004),广东省科委科技计划项目(2007B010600048),芬兰坦佩雷大学科研基金项目(9J114). （2006B50107004）
