中国畜牧兽医2011，Vol.38Issue(2)：243-246,4.
RPMI1640培养基体外培养结肠小袋纤毛虫的研究
Study on the Culture of Balantidium coli in RPMI 1640 Medium in vitro
摘要
Abstract
Development of the vitro culture of Balantidium coli in RPMI 1640 medium is crucial to the further study of the life cycle and pathogenetic mechanism of these intestinal protozoa.The population growth of B.coli under different culture conditions,including concentrations of starch and serum, the initial pH value and various dosages of trophozoites in RPMI 1640 medium were compared.Results indicated that 30 mg/bottle of starch and 20％ serum in the RPMI 1640 medium were appropriate for culturing B.coli.The optimal initial pH value was 7.0.The natural population growth rate and the maximum population density clearly differed with different seeded doses of B.coli.These findings suggested that RPMI 1640 medium could be used to the culture of B.coli.关键词
结肠小袋纤毛虫/RPMI1640培养基/种群自然增长率/体外培养分类
医药卫生引用本文复制引用
王天奇,闫文朝,丁轲,张玲,韩利方,王新彩..RPMI1640培养基体外培养结肠小袋纤毛虫的研究[J].中国畜牧兽医,2011,38(2):243-246,4.基金项目
河南省教育厅自然科学研究计划项目(2009B23003) （2009B23003）
河南科技大学校基金项目(2007ZY042). （2007ZY042）
