中国药理学通报2011，Vol.27Issue(5)：719-722,4.DOI:10.3969/j.issn.1001-1978.2011.05.029
灯盏细辛注射液多成分血浆蛋白结合率测定
Determination of plasma protein binding rate of multicomponent in Dengzhanxixin injection
摘要
Abstract
Aim To establish a detection method for determination of concentration of six major compounds in Dengzhanxixin injection in rat plasma and to study the plasma protein binding rate. Methods The equilibrium dialysis was carried out to determine the plasma protein binding rate of multicomponent in Dengzhanxixin injection , and the drug concentration was detected by HPLC method. The biological samples were all handled with acetic ether-extracting. Results The average plasma protein binding rates of six compounds at low,middle and high concentrations were as follows , respectively : caffeotannic acid was ( 76. 94 ± 3. 72 )％ , caffeic acid was ( 91. 57 ± 0. 96 )％ ,1.5-DCQA was ( 83. 32 ±3. 73 )％ , scutellarin was ( 91. 66 ± 0. 71 )％ .3 ,5DCQA was ( 95. 73 ±0. 67 )％ ,3,4-DCQA was ( 94. 21 ±0. 65 )％. Conclusions The HPLC method is used for the first time to determine the concentration of multicomponent in Dengzhanxixin injection. The method is simple, reliable and stable. The six major compounds in Dengzhanxixin injection have high capacity in binding to plasma protein in rat in vitro.关键词
灯盏细辛注射液/绿原酸/咖啡酸/1,5-DCQA/灯盏乙素/3,5-DCQA/3,4-DCQA/平衡透析法分类
医药卫生引用本文复制引用
周玲,居文政,刘子修,谈恒山..灯盏细辛注射液多成分血浆蛋白结合率测定[J].中国药理学通报,2011,27(5):719-722,4.基金项目
国家重大新药创制资助项目(No 2008ZX09312-004) （No 2008ZX09312-004）
江苏省中医药领军人才资助项目(No LJ200906) （No LJ200906）
