中国药理学通报2011，Vol.27Issue(5)：700-704,5.DOI:10.3969/j.issn.1001-1978.2011.05.024
全反式维甲酸对HFL-Ⅰ细胞α-平滑肌肌动蛋白、Ⅰ型胶原、热休克蛋白47表达的影响
Effect of all-trans-retinoic acid on α-SMA, Collagen-Ⅰ and HSP47 expressions in TGF-β1-stimulated human lung fibroblasts
摘要
Abstract
Aim To investigate the effects of all-transretinoic acid ( ATRA ) on the expression of α-smooth muscle actin( α-SMA ), Collagen-Ⅰ and heat shock protein 47 ( HSP47 ) in the TCF-β1-stimulated human lung fibroblasts ( HFL- Ⅰ ). Methods Firstly, cell proliferation of HFL- Ⅰ was detected by MTT after the cells were treated with different concentrations of TGF β1 and ATRA respectively for 3 days. HFL- Ⅰ cells in vitro were divided into six experimental groups: the control group; 5 μg · L-1 TGF-β1 group; 10 μmol · L-1 ATRA group; 5 μg · L-1 TGF-β1 +0. 1 μmol · L-1 ATRA group; 5 μg · L-1 TCF-β1 +1 μmol · L-1 ATRA group; 5 μg · L-1 TGF-β1 + lOμmol · L-1 ATRA group. Expressions of α-SMA, Collagen- Ⅰ HSP47 were determined through RT-PCR and Western blot. Results Different concentrations of TGF-β1 and ATRA could respectively promote cell proliferation of HFL- Ⅰ in a concentration-dependent manner. Furthermore. in a concentration-dependent manner. ATRA reduced the mRNA and protein expressions of α-SMA. Collagen- Ⅰ and HSP47 which obviously increased in the TGF-β1-stimulated HFL- Ⅰ cells. Conclusions ATRA can inhibit TGF-β1-stimulated differentiation and proliferation of HFL- Ⅰ cells. The mechamsm may be related to the down-regulation of the expression of Collagen- Ⅰ and HSP47.关键词
肌成纤维细胞/转化生长因子-β1/全反式维甲酸/α-平滑肌肌动蛋白/I型胶原/热休克蛋白47分类
医药卫生引用本文复制引用
刘巨源,李佳鑫,海广范,陈永凤,夏武,贾岩龙..全反式维甲酸对HFL-Ⅰ细胞α-平滑肌肌动蛋白、Ⅰ型胶原、热休克蛋白47表达的影响[J].中国药理学通报,2011,27(5):700-704,5.基金项目
河南省教育厅自然科学研究项目(No 2009A310007) （No 2009A310007）
